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Fig. 3 | BMC Plant Biology

Fig. 3

From: The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure and stress-responsive gene expression

Fig. 3

In silico homology model of SAPK9 protein and kinase activity of the recombinant SAPK9 on different substrates. a The model of SAPK9 was constructed by Modeller9.15 (https://salilab.org/modeller/9.15) using Arabidopsis SnRK2.6 (PDB ID: 3UC4) as a template. The SnRK2 box and the activation loop segment are highlighted in cyan and blue, respectively. Predicted phosphorylation sites in activation loop are marked in red (S176, T177) and the Mg2+ binding loop is indicated. b Close view of the catalytic domain, ATP binding loop and phosphorylation site of SAPK9 activation loop. c Coomassie blue stained SDS-PAGE showing E. coli expressed recombinant His-tagged SAPK9 protein purified through Ni-NTA chromatography under native condition. d Autoradiography showing in vitro phosphorylation of histone III substrate with recombinant SAPK9. e Coomassie blue stained SDS-PAGE showing E.coli expressed recombinant His-tagged OsbZIP23 protein purified through Ni-NTA chromatography under native condition. f Autoradiography showing in vitro phosphorylation of OsbZIP23 substrate with recombinant SAPK9

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