Fig. 8

BiPD–overexpressing lines are more tolerant to water stress. a Transgenic lines under a water deficit regime. Arabidopsis plants, genotypes Col–0, BiPDox line T10 and BiPDox line T23 were grown in soil and water stress was induced by withholding irrigation for 20 days. Photography was taken 10 days after withholding irrigation. b Relative water content of wild–type and transgenic leaves exposed to the drought regime. c Expression of AtNRP1 and AtNRP2 during the water stress treatment. Total RNA was isolated from leaves of Col–0 and BiPDox lines at the indicated time points during the water stress treatment. The transcript levels of AtNRP1 were quantified by qRT–PCR. Gene expression was calculated using the 2^-ΔΔCt method and Actin as endogenous control. cDNAs were obtained from five biological replicates and validated individually. Error bars indicate the 95 % confidence interval based on a t-test (p < 0,05)