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Fig. 1 | BMC Plant Biology

Fig. 1

From: BOTRYTIS-INDUCED KINASE1, a plasma membrane-localized receptor-like protein kinase, is a negative regulator of phosphate homeostasis in Arabidopsis thaliana

Fig. 1

Responsiveness of BIK1 to Pi starvation. a Expression changes of BIK1 in different tissues of WT plants under + Pi and –Pi conditions. Seven-day-old seedlings grown hydroponically under normal Pi condition were transferred to medium supplemented with Pi (+Pi, 250 μM) or without Pi (−Pi). Roots, shoots and leaves were collected for analysis of BIK1 expression by qRT-PCR at indicated time points after transferring. b Detection of BIK1 promoter activity in BIK1 pro ::GUS seedlings by GUS staining. c Expression changes of GUS gene in root and lead tissues of BIK1 pro ::GUS seedlings under –Pi condition. Seven-day-old BIK1 pro ::GUS seedlings grown in normal medium were transferred to medium without Pi supplement and samples were collected at 0 and 1 day after transferring for GUS staining and analysis of gene expression. Data were normalized with the transcript level of UBQ10 and relative expression levels were shown as folds of the UBQ10 level. Data presented are the means ± SD from three independent experiments and * above the error bars indicate significant differences at p < 0.05 level between the + Pi and –Pi conditions

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