Fig. 5

HPLC analysis of carotenoids in β-carotene-accumulating E. coli BL21 (DE3) cells transformed with pRSF-2 Ek/LIC-CitHYb (b), pRSF-2 Ek/LIC-CitCYP97A (c), pRSF-2 Ek/LIC-CitCYP97B (d), and pRSF-2 Ek/LIC-CitCYP97C (e). Carotenoids extracted from the suspension cultures of β-carotene-accumulating E. coli BL21 (DE3) cells with pRSF-2 (empty vector) were used as control (a). β-Car, β-carotene; β-Cry, β-cryptoxanthin; Zea, zeaxanthin