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Fig. 5 | BMC Plant Biology

Fig. 5

From: Expression of B-class MADS-box genes in response to variations in photoperiod is associated with chasmogamous and cleistogamous flower development in Viola philippica

Fig. 5

The floral expression of B-class MADS-box genes in response to variations in photoperiod. ac Relative expression of VpTM6-1, VpTM6-2, and VpPI in flower buds and mature flowers. a VpTM6-1. b VpTM6-2. c VpPI. Gene expression in flower buds around the third stage (Fl1), the fourth to fifth stages (Fl2), mature flowers (Mf) and leaves (Le) under 10- (open columns), 12- (gray columns) and 16-h (black columns) daylight were evaluated using qRT-PCR. The gene expression in Fl1 under 10-h daylight was set to 1. df Relative expression of B-class MADS-box genes in floral organs of mature flowers. d VpTM6-1. e VpTM6-2. f VpPI. Gene expression in stamens (open columns), petals (black columns), and pistils (gray columns) from CH, inCL, and CL flowers under the indicated photoperiods were measured using qRT-PCR. No visible expression signal was detected in sepals under various conditions. # indicates poorly developed stamens in inCL flowers. Gray “CL” indicates that the petal was not collected from the CL flowers. The level of gene expression in the stamens of CH flowers subjected to 10-h daylight was set to 1. Three independent biological samples were used in all these analyses. Mean and standard errors are presented

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