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Table 3 Copper and cadmium content in A. thaliana wildtype and spl7 knockout plants

From: Toxicity responses of Cu and Cd: the involvement of miRNAs and the transcription factor SPL7

   

24 h

72 h

   

WT

spl7

WT

spl7

Cu

leaves

Control

7.50 ± 0.53abc

6.53 ± 0.37bc

8.95 ± 0.81abe

9.76 ± 0.66ade

2 μM Cu

12.43 ± 0.32d

11.63 ± 0.44de

16.70 ± 1.33f

16.79 ± 0.74f

5 μM Cd

6.31 ± 0.21bc

4.93 ± 0.74c

6.82 ± 0.17abc

4.64 ± 0.48c

roots

Control

25.00 ± 2.81abc

25.61 ± 1.93abc

18.91 ± 1.18c

23.47 ± 2.24bc

2 μM Cu

1069.45 ± 30.28d

712.47 ± 38.00e

943.04 ± 43.13de

854.71 ± 50.80de

5 μM Cd

29.97 ± 2.50ab

27.38 ± 2.18ab

45.85 ± 1.71f

34.45 ± 2.04af

Cd

leaves

5 μM Cd

514.45 ± 6.05a

699.38 ± 156.61a

1831.70 ± 53.69b

1549.40 ± 151.09b

roots

5 μM Cd

884.79 ± 116.14a

1282.86 ± 152.62a

2421.08 ± 163.42b

3034.21 ± 113.16c

  1. Nineteen-days-old plants were exposed for 24 or 72 h to 2 μM CuSO4, 5 μM CdSO4 or grown under control conditions. Copper and Cd content (mg kg-1 DW-1) in roots and leaves were calculated. Data are given as the mean ± S.E. of 5 biological independent replicates. Significant differences (P < 0.05) after three-way ANOVA test (Cu content) or two-way ANOVA test (Cd content; time/genotype) and Tukey correction are indicated with different small letters (per metal, per organ)