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Table 4 Disease Severity lsmeans scores and A. euteiches DNA amounts in roots, in multiple-QTL NIL experiments

From: Single and multiple resistance QTL delay symptom appearance and slow down root colonization by Aphanomyces euteiches in pea near isogenic lines

Experiment (Strain)

Crossa

Genotype

QTLb

Symptom scoringc

A. euteiches DNA amounte

Day after inoculation

Day after inoculation

2d

3d

4f

5

6

7

10

2

3

4

5

6

7f

10f

3

DSP * RIL 847.50

NIL4-0b

-

NA

NA

−1.6

−2.9

−1.2

−4.6

−5.0

NA

23.8 ± 2.5

226.5 ± 14.1

522.6 ± 23.8

696.1 ± 36.8

817.6 ± 41.7

1142.9 ± 59.4

(RB84)

 

NIL4-5.1a

Ae-Ps5.1

NA

NA

−0.7

−1.5

−0.1

−2.4***

−4.6

NA

18.1 ± 2.5

131.8 ± 14.1***

368 ± 21.3***

558.4 ± 33.8**

668.1 ± 35.1*

871.7 ± 59.4***

  

NIL4-7.6ag

Ae-Ps7.6

NA

NA

0.5*

0.0***

1.0**

−1.5

−3.8

NA

11.9 ± 3.1**

98.1 ± 17.2***

339.3 ± 28.1***

561.8 ± 42*

633.1 ± 41.7*

855.8 ± 71.8***

  

NIL4-5.1/7.6a

Ae-Ps5.1 + Ae-Ps7.6

NA

NA

1.9***

0.9***

3.5***

0.5***

−0.1***

NA

7 ± 2.5***

73.8 ± 15.2***

155.7 ± 21.3***

297.9 ± 33.8***

477.9 ± 31.5***

826.2 ± 59.4***

  

NIL4-5.1/7.6b

Ae-Ps5.1 + Ae-Ps7.6

NA

NA

1.2***

0.8***

3.4***

1.3***

−1.4**

NA

9.1 ± 2.5***

71.1 ± 14.1***

210.6 ± 21.3***

363.3 ± 36.7***

495.1 ± 31.5***

739.1 ± 59.4***

  

90-2131

-

NA

NA

−0.3

−1.7

−0.8

−3.3

−4.3

NA

15.3 ± 2.5*

101.5 ± 14.1***

279.4 ± 21.3***

533.8 ± 33.8***

675.2 ± 35.4

911 ± 59.4***

  

RIL 847.50

-

NA

NA

1.2***

1.0***

2.5***

−0.2***

−2.0*

NA

7.6 ± 2.5***

45.4 ± 14.1***

170.1 ± 21.3***

NA

380 ± 35.4***

758.3 ± 59.4***

4

Baccara * 552

NIL7-0b

-

NA

NA

0.6

2.0

2.8

5.0

2.7

1.3 ± 0.3

6.9 ± 0.9

70.5 ± 7.9

295.3 ± 38.3

634.7 ± 52.9

806.1 ± 56.1

1948 ± 156.1

(RB84)

 

NIL13-2.2a

Ae-Ps2.2

NA

NA

−0.9

0.9

2.3

4.5

2.0

1.6 ± 0.2

12.3 ± 0.9

86.7 ± 7.7

309 ± 36

798.5 ± 50.3

970.4 ± 64.3

2095.5 ± 156.1

  

NIL13-3.1a

Ae-Ps3.1

NA

NA

−1.3*

0.1*

1.0

3.2

1.7

3.6 ± 0.6

6 ± 1.1

42.5 ± 7.7*

270 ± 35.5

455.6 ± 43.8

761.6 ± 56.1

1384.5 ± 167.9

  

NIL13-7.6b

Ae-Ps7.6

NA

NA

−2.0***

−0.4***

1.4

2.9*

3.1

NA

3.3 ± 1.3***

28.9 ± 7.7***

239.1 ± 35.5

476.2 ± 53

709.9 ± 56.1

1544.2 ± 156.1

  

NIL13-2.2/7.6a

Ae-Ps2.2 + Ae-Ps7.6

NA

NA

−1.2**

0.6

1.4

3.1

3.0

NA

4.7 ± 0.9

55.8 ± 7.9

314.6 ± 35.5

566.9 ± 45.5

805.5 ± 64.3

1573.8 ± 156.1

  

NIL13-2.2/7.6b

Ae-Ps2.2 + Ae-Ps7.6

NA

NA

−1.1

0.7

1.8

3.5

2.0

NA

6.8 ± 0.9

66.3 ± 7.9

346.7 ± 37.4

651.1 ± 43.8

901.2 ± 58.3

1416.7 ± 167.9

  

NIL13-3.1/7.6a

Ae-Ps3.1 + Ae-Ps7.6

NA

NA

−1.8

−0.8***

0.8

2.3**

1.5

1.1 ± 0.7

3 ± 1***

35.7 ± 7.9**

166.5 ± 36**

431.1 ± 45.5**

694.5 ± 56.1

1254.7 ± 156.1**

  

NIL13-3.1/7.6b

Ae-Ps3.1 + Ae-Ps7.6

NA

NA

−2.1

−0.4**

0.9

2.4**

2.5

1.1 ± 0.6

3.4 ± 0.9

46.7 ± 7.9*

259.3 ± 35.5

494 ± 43.8

680 ± 56.1

1522 ± 156.1

  

NIL13-2.2/3.1/7.6a

Ae-Ps2.2 + Ae-Ps3.1 + Ae-Ps7.6

NA

NA

−2.0***

−0.8***

0.9

2.8*

0.9

1.1 ± 0.7

3.1 ± 1.1**

30.9 ± 7.7***

181.8 ± 36*

428.4 ± 43.8*

576.2 ± 64.3*

1231 ± 156.1**

  

NIL13-2.2/3.1/7.6b

Ae-Ps2.2 + Ae-Ps3.1 + Ae-Ps7.6

NA

NA

−1.2**

−0.1*

0.6

3.0

2.9

NA

3.8 ± 0.9**

42.3 ± 7.7*

221.4 ± 36

403.7 ± 43.8**

646.9 ± 64.3

1258.8 ± 161.6*

  

552

-

NA

NA

−2.9***

−2.7***

−2.5***

−1.9***

0.3*

NA

1.1 ± 1.5***

3.7 ± 8.5***

41.4 ± 35.5***

127.5 ± 43.8***

286.7 ± 56.1***

703.7 ± 156.1***

  1. a“Recipient x donor” cross lines from which each NIL was produced in the previous MAB scheme [25]. bQTL introgressed in each NIL from the previous MAB scheme [25]. cLSMeans disease severity (DS) scores obtained on each genotype and scoring day from the CLMM analysis of each NIL set in experiments #3 and #4. Lsmeans scores were obtained from the DS score probabilities for each genotype and scoring day represented in Additional file 1B. LSMeans DS values ranged from −5 to 5, according to the scale of the latent variable implied by the CLMM. Significant differences between LSMeans values of the single-QTL NILs or the resistant control, and the control-NIL without QTL are indicated by *(0.01 < P < 0.05), **(0.001 < P < 0.01) and ***(P < 0.001). dAt two and three days after inoculation, LSMeans DS scores could not be estimated from CLMM since some lines did not have symptoms. ePathogen DNA amount were obtained on each genotype and scoring day from the LMM analysis of each NILs set in experiments #3 and #4. LSMeans and standard errors of pathogen DNA amount are presented in thousand DNA copies (10^3). In each set of lines, significant differences of LSMeans DNA amount between the single-QTL NILs or the resistant control, and the control- NIL without QTL are indicated by *(0.01 < P < 0.05), **(0.001 < P < 0.01) and ***(P < 0.001). fLSMeans scores were estimated from data in one biological replicate, for DS scoring at the fourth day in experiment #3 and #4 and for pathogen DNA data at the tenth day in experiment #3 and the seventh day in experiment #4. gLSMeans DS scores and pathogen DNA amounts for NIL4-7.6a were estimated from data in one biological replicate at all days. NA: Not Available data due to copy number <103 or to inconsistent data. In bold, significant differences of LSMeans DS scores or DNA amounts between the multiple-QTL NILs and the single-QTL NIL NIL4-7.6a for experiment #3 or NIL13-7.6b for experiment #4 (P < 0.05)
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BMC Plant Biology

ISSN: 1471-2229

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