Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

Huang, Ying; Ma, Hong-Yu; Huang, Wei; Wang, Feng; Xu, Zhi-Sheng; Xiong, Ai-Sheng

doi:10.1186/s12870-016-0819-z

Table 1 A total of 86 differentially expressed proteins identified in resistant and susceptible tomato cultivars after TYLCV infection

From: Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

^aSpot no.	^bAccession no.	^cProtein name, Orgnaism	^dProtein score	^eSC	^fMP	^gMW (kDa) /pI	^hZ_C/J_C	^hZ_T/J_T	^hZ_T/Z_C	^hJ_T/J_C
Photosynthesis
1	gi\|407970998	Chlorophyll a-b binding protein 4, Solanum lycopersicum	135	13	2	28.98/5.33	0.31^* ± 0.15	0.45^* ± 0.35	0.22^* ± 0.13	0.18^* ± 0.09
2	gi\|115813	Chlorophyll a-b binding protein 8, Solanum lycopersicum	212	15	5	29.34/8.96	1.09 ± 0.22	0.45^* ± 0.35	0.25^* ± 0.06	0.90 ± 0.33
3	gi\|460405507	Chlorophyll a-b binding protein 8, Solanum lycopersicum	81	4	1	29.26/8.65	1.34 ± 0.25	1.78 ± 0.30	0.46^* ± 0.03	0.36^* ± 0.13
4	gi\|460375240	Oxygen-evolving enhancer protein 1, Solanum lycopersicum	273	10	2	35.15/5.91	0.84 ± 0.07	0.18^* ± 0.01	0.31^* ± 0.04	1.49 ± 0.38
5	gi\|460372520	Oxygen-evolving enhancer protein 1, Solanum lycopersicum	99	19	2	35.48/5.84	1.93 ± 0.19	0.18^* ± 0.01	2.21^* ± 0.44	10.57^* ± 0.52
6	gi\|460408969	Rubisco accumulation factor 1, Solanum lycopersicum	119	9	3	50.46/5.10	5.36^* ± 0.28	1.85 ± 0.28	0.77 ± 0,29	2.18^* ± 0.39
7	gi\|460401823	Ribulose bisphosphate carboxylase/oxygenase activase 1, Solanum lycopersicum	56	3	1	49.05/8.15	0.09^* ± 0.04	0.57 ± 0.12	6.75^* ± 0.18	1.07 ± 0.29
8	gi\|723739979	Ribulose bisphosphate carboxylase/oxygenase activase, Solanum lycopersicum	285	11	3	50.97/8.76	0.88 ± 0.20	0.38^* ± 0.30	0.07^* ± 0.05	0.16^* ± 0.01
9	gi\|1778414	Ribulose 1,5-bisphosphate carboxylase/oxygenase activase, Oryza sativa	126	7	2	48.06/5.85	0.39^* ± 0.04	0.78 ± 0.16	3.45^* ± 0.40	1.73 ± 0.01
10	gi\|100380	Ribulose bisphosphate carboxylase activase, Nicotiana tabacum	103	7	1	26.08/5.01	0.05^* ± 0.01	0.03^* ± 0.02	3.25^* ± 0.44	5.57^* ± 0.43
11	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum	171	6	3	48.57/6.71	1.22 ± 0.14	43.48^* ± 0.38	0.04^* ± 0.01	0.01^* ± 0,01
12	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum	313	13	5	48.57/6.71	1.58 ± 0.11	23.71^* ± 0.28	0.06^* ± 0.01	0.02^* ± 0.01
13	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum	62	4	2	48.57/6.71	1.37 ± 0.22	4.53^* ± 0.25	0.05^* ± 0.01	0.05^* ± 0.01
14	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum	124	8	3	48.57/6.71	0.83 ± 0.15	1.89 ± 0.20	0.09^* ± 0.01	0.04^* ± 0.01
15	gi\|1293000	Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Cheirodendron trigynum	200	6	3	52.90/6.31	0.63 ± 0.04	1.18 ± 0.20	0.10^* ± 0.05	0.06^* ± 0.01
16	gi\|168282	Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Lactoris fernandeziana	281	16	4	44.16/6.33	0.19^* ± 0.02	0.28^* ± 0.02	2.60^* ± 0.39	1.76 ± 0.24
17	gi\|21069067	Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Asplenium jahandiezii	60	4	1	39.38/7.31	1.00 ± 0.26	0.29^* ± 0.10	0.02^* ± 0.01	0.09^* ± 0.08
18	gi\|460379814	RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum	203	10	4	64.53/5.46	5.11^* ± 0.34	2.87^* ± 0.15	1.26 ± 0.06	2.24^* ± 0.19
19	gi\|460366131	RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum	53	4	2	63.24/5.72	1.15 ± 0.36	2.81^* ± 0.25	0.69 ± 0.08	0.28^* ± 0.09
20	gi\|92087012	Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum	65	6	3	53.43/6.55	0.77 ± 0.12	10.02^* ± 0.24	0.09^* ± 0.01	0.01^* ± 0.01
21	gi\|92087012	Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum	121	14	6	53.43/6.55	1.18 ± 0.07	32.57^* ± 0.19	0.08^* ± 0.01	0.01^* ± 0.01
22	gi\|460375527	Ferredoxin–NADP reductase, leaf-type isozyme, Solanum lycopersicum	118	13	3	40.90/8.67	0.28^* ± 0.07	0.93 ± 0.47	0.42^* ± 0.10	0.14^* ± 0.02
23	gi\|460373374	FerredoxinvNADP reductase, leaf-type isozyme, Solanum lycopersicum	299	20	5	40.77/8.37	0.82 ± 0.07	0.61 ± 0.07	0.48^* ± 0.02	0.65 ± 0.05
24	gi\|350537679	Carbonic anhydrase, Solanum lycopersicum	278	17	4	34.85/6.67	1.45 ± 0.12	3.21^* ± 0.27	0.85 ± 0.12	0.38^* ± 0.05
25	gi\|350537679	Carbonic anhydrase, Solanum lycopersicum	98	5	1	34.85/6.67	0.65 ± 0.15	0.35^* ± 0.03	1.53 ± 0.18	2.81^* ± 0.23
26	gi\|460389468	Protein TIC 62, Solanum lycopersicum	313	13	5	52.96/7.66	1.02 ± 0.14	0.20^* ± 0.11	0.13^* ± 0.04	0.70 ± 0.17
27	gi\|460372959	Peptidyl-prolyl cis-trans isomerase CYP38, Solanum lycopersicum	185	17	5	49.54/5.00	0.92 ± 0.12	1.61 ± 0.38	0.26^* ± 0.05	0.15^* ± 0.04
Carbohydrate metabolism and energy
28	gi\|469517896	Glyceraldehyde-3-phosphate dehydrogenase, Atropa belladonna	101	10	4	34.16/6.04	0.88 ± 0.11	0.98 ± 0.29	0.46^* ± 0.03	0.44^* ± 0.13
29	gi\|460414390	ATP synthase delta chain, Solanum lycopersicum	166	6	4	27.32/8.90	0.49^* ± 0.17	1.62 ± 0.23	0.38^* ± 0.05	0.11^* ± 0.03
30	gi\|303279681	Aryl-al/cohol dehydrogenase related protein, Micromonas pusilla	54	3	1	41.07/8.65	0.53 ± 0.11	1.05 ± 0.21	2.49^* ± 0.35	1.28 ± 0.19
31	gi\|460397188	Thiamine thiazole synthase, Solanum lycopersicum	142	14	4	37.68/5.42	0.30^* ± 0.21	3.06^* ± 0.09	2.25^* ± 0.07	0.23^* ± 0.17
32	gi\|460365268	Biotin carboxylase 1, Solanum lycopersicum	413	13	6	59.08/6.52	0.68 ± 0.27	1.51 ± 0.26	0.82 ± 0.08	0.37^* ± 0.13
33	gi\|723747143	Aconitate hydratase, Solanum lycopersicum	218	5	4	107.83/6.52	67.86^* ± 0.19	3.13^* ± 0.22	0.26^* ± 0.02	5.77^* ± 0.85
34	gi\|350538543	Succinic semialdehyde reductase isofom 2, Solanum lycopersicum	123	7	2	38.94/8.50	2.01^* ± 0.26	2.52^* ± 0.10	1.24 ± 0.09	1.06 ± 0.93
35	gi\|460408278	Enolase-like, Solanum lycopersicum	207	11	4	48.21/5.99	3.37^* ± 0.29	4.38^* ± 0.04	1.49 ± 0.09	1.14 ± 0.14
36	gi\|460415839	Probable ATP synthase 24 kDa subunit, Solanum lycopersicum	78	5	1	27.75/8.69	0.44^* ± 0.08	0.49^* ± 0.12	1.31 ± 0.09	1.02 ± 0.28
37	gi\|460373820	ATP synthase gamma chain, Solanum lycopersicum	167	12	3	41.75/8.15	1.93 ± 0.33	3.80^* ± 0.24	0.09^* ± 0.01	0.05^* ± 0.01
38	gi\|460365435	Isocitrate dehydrogenase [NADP], Solanum lycopersicum	172	12	4	47.00/6.35	1.05 ± 0.22	0.27^* ± 0.12	0.21^* ± 0.03	0.86 ± 0.14
39	gi\|350535679	Cytosolic NADP-malic enzyme, Solanum lycopersicum	167	6	3	64.63/5.71	2.04^* ± 0.20	1.20 ± 0.14	0.48^* ± 0.07	0.49^* ± 0.20
40	gi\|31088232	Chitinase, Solanum lycopersicum	123	18	7	28.05/5.93	0.82 ± 0.11	0.30^* ± 0.03	1.51 ± 0.31	4.12^* ± 0.10
Proteometabolism
41	gi\|350535160	Wound-inducible carboxypeptidase precursor, Solanum lycopersicum	191	5	2	56.04/5.84	1.14 ± 0.07	0.78 ± 0.05	3.62^* ± 0.30	5.27^* ± 0.22
42	gi\|350535160	Wound-inducible carboxypeptidase precursor, Solanum lycopersicum	177	5	3	56.04/5.84	1.07 ± 0.17	0.48^* ± 0.02	2.72^* ± 0.19	6.05^* ± 0.30
43	gi\|350534564	26S protease regulatory subunit 6A homolog, Solanum lycopersicum	253	17	6	47.70/4.94	1.18 ± 0.05	0.78 ± 0.25	0.20^* ± 0.07	0.30^* ± 0.04
44	gi\|460393754	26S protease regulatory subunit 6B homolog, Solanum lycopersicum	198	7	2	46.80/5.63	1.24 ± 0.40	0.38^* ± 0.13	0.33^* ± 0.23	0.91 ± 0.34
45	gi\|460400419	Elongation factor G, Solanum lycopersicum	258	11	5	86.79/5.45	3.75^* ± 0.31	0.53 ± 0.02	0.47^* ± 0.01	3.29^* ± 0.39
46	gi\|460415494	Eukaryotic initiation factor 4A-2, Solanum lycopersicum	370	18	7	47.10/5.46	1.82 ± 0.06	1.72 ± 0.15	0.35^* ± 0.02	0.37^* ± 0.02
47	gi\|460399092	Eukaryotic initiation factor 4A-2, Solanum lycopersicum	304	13	4	47.14/5.54	1.22 ± 0.22	2.73^* ± 0.07	0.49^* ± 0.22	0.22^* ± 0.05
48	gi\|460391817	Elongation factor TuB, Solanum lycopersicum	125	5	2	56.29/6.69	0.62 ± 0.16	4.33^* ± 0.23	2.45^* ± 0.23	0.34^* ± 0.03
49	gi\|460399098	Elongation factor 2 isoform X1, Solanum lycopersicum	248	7	4	94.97/5.84	1.35 ± 0.24	3.39^* ± 0.23	3.06^* ± 0.11	1.22 ± 0.19
50	gi\|460396224	Eukaryotic translation initiation factor 3 subunit K, Solanum lycopersicum	134	11	2	26.55/5.28	0.46^* ± 0.03	1.12 ± 0.17	2.80^* ± 0.26	1.15 ± 0.12
51	gi\|460391351	Cell division cycle protein 48 homolog, Solanum lycopersicum	172	9	5	90.15/5.10	24.39^* ± 0.39	1.57 ± 0.13	0.38^* ± 0.04	5.90^* ± 0.81
52	gi\|460411520	Cell division cycle protein 48 homolog, Solanum lycopersicum	138	5	3	90.25/5.20	5.85^* ± 0.22	0.91 ± 0.08	1.09 ± 0.04	7.00^* ± 0.60
53	gi\|2492530	Leucine aminopeptidase 2, Solanum lycopersicum	79	8	3	60.08/8.18	4.80^* ± 0.15	2.68^* ± 0.29	0.81 ± 0.07	1.46 ± 0.07
54	gi\|350536267	Subtilisin-like protease precursor, Solanum lycopersicum	301	5	3	79.57/6.25	0.24^* ± 0.02	0.07^* ± 0.01	4.17^* ± 0.29	14.46^* ± 0.40
55	gi\|460381101	28 kDa ribonucleoprotein, Solanum lycopersicum	100	8	3	32.69/4.70	1.11 ± 0.19	1.15 ± 0.31	2.17^* ± 0.70	2.05^* ± 0.25
Amino acid metabolism
56	gi\|723717714	Phosphoglycerate kinase, Solanum lycopersicum	275	17	6	42.26/5.78	1.24 ± 0.46	0.47^* ± 0.02	0.42^* ± 0.02	1.11 ± 0.41
57	gi\|460404838	Adenosylhomocysteinase, Solanum lycopersicum	119	12	4	53.59/5.57	1.10 ± 0.34	0.53 ± 0.16	0.21^* ± 0.06	0.45^* ± 0.19
58	gi\|460395681	Ketol-acid reductoisomerase, Solanum lycopersicum	68	5	2	64.19/6.31	0.83 ± 0.40	0.34^* ± 0.20	0.39^* ± 0.09	0.97 ± 0.21
59	gi\|460415192	S-adenosylmethionine synthase 2, Solanum lycopersicum	207	7	2	43.51/5.41	2.09^* ± 0.26	2.04^* ± 0.07	0.64 ± 0.06	0.86 ± 0.09
60	gi\|460399143	Glycine dehydrogenase (decarboxylating), Solanum lycopersicum	192	9	6	114.02/6.69	1.90 ± 0.14	1.39 ± 0.08	4.02^* ± 0.25	5.51^* ± 0.19
61	gi\|460399143	Glycine dehydrogenase (decarboxylating), Solanum lycopersicum	153	7	5	114.02/6.69	3.80^* ± 0.19	5.08^* ± 0.31	6.81^* ± 0.38	5.09^* ± 0.21
62	gi\|460370413	Glycine dehydrogenase, Solanum lycopersicum	183	9	3	42.52/6.56	0.28^* ± 0.02	12.57^* ± 0.28	3.33^* ± 0.16	0.07^* ± 0.02
63	gi\|170458	Threonine deaminase, partial, Solanum lycopersicum	194	9	4	65.05/5.26	1.01 ± 0.01	0.82 ± 0.04	5.90^* ± 0.09	7.29^* ± 0.29
64	gi\|170458	Threonine deaminase, partial, Solanum lycopersicum	443	18	7	65.05/5.26	0.22^* ± 0.01	0.40^* ± 0.02	5.28^* ± 0.62	2.96^* ± 0.28
65	gi\|170458	Threonine deaminase, partial, Solanum lycopersicum	175	7	3	65.05/5.26	0.97 ± 0.01	0.55 ± 0.02	4.35^* ± 0.03	7.62^* ± 0.14
66	gi\|460398434	Cysteine synthase, Solanum lycopersicum	317	20	4	41.26/5.41	0.99 ± 0.02	6.39^* ± 0.21	2.11^* ± 0.22	0.33^* ± 0.04
67	gi\|460404180	Cysteine synthase, Solanum lycopersicum	274	17	5	34.34/5.93	1.89 ± 0.13	0.63 ± 0.38	0.33^* ± 0.16	1.09 ± 0.19
Chaperones
68	gi\|460389504	Protein disulfide-isomerase-like, Solanum lycopersicum	82	6	2	55.11/4.81	0.53 ± 0.02	0.55 ± 0.01	3.51^* ± 0.28	3.39^* ± 0.22
69	gi\|460395973	Heat shock protein 83, Solanum lycopersicum	76	1	1	90.77/5.23	0.96 ± 0.04	1.99 ± 0.03	19.73^* ± 0.24	9.53^* ± 0.23
70	gi\|460395973	Heat shock protein 83, Solanum lycopersicum	193	3	3	90.77/5.23	1.24 ± 0.23	3.10^* ± 0.37	2.07^* ± 0.26	0.82 ± 0.07
71	gi\|762844	Hsc70, Solanum lycopersicum	115	4	2	71.87/5.18	5.38^* ± 0.40	0.60 ± 0.15	1.04 ± 0.28	9.20^* ± 0.21
72	gi\|170386	Glucose-regulated protein 78, Solanum lycopersicum	211	12	3	41.32/8.51	2.45^* ± 0.40	0.73 ± 0.14	1.31 ± 0.26	4.32^* ± 0.41
73	gi\|145341034	AAA-metalloprotease FtsH, Ostreococcus lucimarinus	131	6	4	67.77/5.23	0.92 ± 0.05	0.63 ± 0.29	0.44^* ± 0.01	0.75 ± 0.32
Signal transduction
74	gi\|1168191	14-3-3 protein 4 OS, Solanum lycopersicum	58	25	4	29.40/4.69	0.78 ± 0.33	0.67 ± 0.29	0.10^* ± 0.04	0.11^* ± 0.05
75	gi\|3041662	14-3-3 protein 3, Solanum lycopersicum	165	12	2	29.40/4.74	0.56 ± 0.23	1.33 ± 0.24	0.21^* ± 0.04	0.08^* ± 0.02
76	gi\|460405902	Plasma membrane-associated cation-binding protein 1, Solanum lycopersicum	95	6	1	21.98/5.03	1.71 ± 0.37	0.99 ± 0.19	0.20^* ± 0.04	0.34^* ± 0.04
77	gi\|590715109	Transducin family protein/WD-40 repeat family protein isoform 1, Theobroma cacao	57	2	1	87.16/6.40	0.82 ± 0.01	0.18^* ± 0.01	2.87^* ± 0.03	12.76^* ± 0.11
78	gi\|460393840	Proliferation-associated protein 2G4-like, Solanum lycopersicum	134	7	2	43.07/6.41	2.16^* ± 0.17	0.59 ± 0.15	0.27^* ± 0.09	0.97 ± 0.19
79	gi\|525314284	Hop-interacting protein THI113, Solanum lycopersicum	117	10	2	37.34/5.82	0.93 ± 0.14	0.11^* ± 0.07	0.24^* ± 0.18	1.96 ± 0.10
Detoxification and antioxidation
80	gi\|350536897	Cytosolic ascorbate peroxidase 1, Solanum lycopersicum	113	14	2	27.73/5.61	1.54 ± 0.26	0.66 ± 0.06	0.42^* ± 0.09	0.96 ± 0.09
81	gi\|350536897	Cytosolic ascorbate peroxidase 1, Solanum lycopersicum	115	7	1	27.73/5.61	7.20^* ± 0.13	7.42^* ± 0.32	6.03^* ± 0.16	5.85^* ± 0.07
82	gi\|350539113	Ascorbate peroxidase, Solanum lycopersicum	141	5	2	42.38/8.65	1.90 ± 0.20	0.75 ± 0.18	0.32^* ± 0.04	0.81 ± 0.07
83	gi\|460373807	Putative lactoylglutathione lyase, Solanum lycopersicum	126	15	5	32.95/5.95	0.82 ± 0.22	0.17^* ± 0.07	0.44^* ± 0.21	2.05^* ± 0.24
84	gi\|50400860	Monodehydroascorbate reductase, Solanum lycopersicum	255	17	5	47.12/5.77	0.85 ± 0.07	0.49^* ± 0.17	0.40^* ± 0.18	0.69 ± 0.16
85	gi\|251895	Polyphenol oxidase, Solanum lycopersicum	169	7	5	66.83/6.61	1.96 ± 0.06	2.87^* ± 0.04	15.32^* ± 0.10	10.49^* ± 0.42
86	gi\|460397526	Heme-binding protein 2, Solanum lycopersicum	183	17	3	26.12/7.59	0.84 ± 0.08	0.82 ± 0.05	4.29^* ± 0.25	4.41^* ± 0.18

^aNumbering corresponds to the 2-DE in Fig. 3
^bAccession number from the NCBI nr database
^cNames and species of the proteins obtained via the MASCOT software from the NCBI nr database
^dMolecular weight search (MOWSE) score probability for the entire protein identified by the MASCOT software
^eThe sequence coverage of identified proteins
^fTotal numbers of identified peptides
^gMolecular weight and isoelectric point of the identified proteins
^hProtein abundance ratio of Treatment/Control tomato cultivars, with each value representing the mean value ± SD of three biological replicates
^∗Indicates significant (more than 2.0-fold or less than 0.50-fold) difference between control and treatment tomato cultivars

Back to article page

ISSN: 1471-2229

Contact us

Submission enquiries: bmcplantbiology@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Plant Biology

Contact us