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Table 1 A total of 86 differentially expressed proteins identified in resistant and susceptible tomato cultivars after TYLCV infection

From: Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

aSpot no. bAccession no. cProtein name, Orgnaism dProtein score eSC fMP gMW (kDa) /pI hZC/JC hZT/JT hZT/ZC hJT/JC
Photosynthesis
 1 gi|407970998 Chlorophyll a-b binding protein 4, Solanum lycopersicum 135 13 2 28.98/5.33 0.31* ± 0.15 0.45* ± 0.35 0.22* ± 0.13 0.18* ± 0.09
 2 gi|115813 Chlorophyll a-b binding protein 8, Solanum lycopersicum 212 15 5 29.34/8.96 1.09 ± 0.22 0.45* ± 0.35 0.25* ± 0.06 0.90 ± 0.33
 3 gi|460405507 Chlorophyll a-b binding protein 8, Solanum lycopersicum 81 4 1 29.26/8.65 1.34 ± 0.25 1.78 ± 0.30 0.46* ± 0.03 0.36* ± 0.13
 4 gi|460375240 Oxygen-evolving enhancer protein 1, Solanum lycopersicum 273 10 2 35.15/5.91 0.84 ± 0.07 0.18* ± 0.01 0.31* ± 0.04 1.49 ± 0.38
 5 gi|460372520 Oxygen-evolving enhancer protein 1, Solanum lycopersicum 99 19 2 35.48/5.84 1.93 ± 0.19 0.18* ± 0.01 2.21* ± 0.44 10.57* ± 0.52
 6 gi|460408969 Rubisco accumulation factor 1, Solanum lycopersicum 119 9 3 50.46/5.10 5.36* ± 0.28 1.85 ± 0.28 0.77 ± 0,29 2.18* ± 0.39
 7 gi|460401823 Ribulose bisphosphate carboxylase/oxygenase activase 1, Solanum lycopersicum 56 3 1 49.05/8.15 0.09* ± 0.04 0.57 ± 0.12 6.75* ± 0.18 1.07 ± 0.29
 8 gi|723739979 Ribulose bisphosphate carboxylase/oxygenase activase, Solanum lycopersicum 285 11 3 50.97/8.76 0.88 ± 0.20 0.38* ± 0.30 0.07* ± 0.05 0.16* ± 0.01
 9 gi|1778414 Ribulose 1,5-bisphosphate carboxylase/oxygenase activase, Oryza sativa 126 7 2 48.06/5.85 0.39* ± 0.04 0.78 ± 0.16 3.45* ± 0.40 1.73 ± 0.01
 10 gi|100380 Ribulose bisphosphate carboxylase activase, Nicotiana tabacum 103 7 1 26.08/5.01 0.05* ± 0.01 0.03* ± 0.02 3.25* ± 0.44 5.57* ± 0.43
 11 gi|488453358 Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum 171 6 3 48.57/6.71 1.22 ± 0.14 43.48* ± 0.38 0.04* ± 0.01 0.01* ± 0,01
 12 gi|488453358 Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum 313 13 5 48.57/6.71 1.58 ± 0.11 23.71* ± 0.28 0.06* ± 0.01 0.02* ± 0.01
13 gi|488453358 Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum 62 4 2 48.57/6.71 1.37 ± 0.22 4.53* ± 0.25 0.05* ± 0.01 0.05* ± 0.01
 14 gi|488453358 Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum 124 8 3 48.57/6.71 0.83 ± 0.15 1.89 ± 0.20 0.09* ± 0.01 0.04* ± 0.01
 15 gi|1293000 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Cheirodendron trigynum 200 6 3 52.90/6.31 0.63 ± 0.04 1.18 ± 0.20 0.10* ± 0.05 0.06* ± 0.01
 16 gi|168282 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Lactoris fernandeziana 281 16 4 44.16/6.33 0.19* ± 0.02 0.28* ± 0.02 2.60* ± 0.39 1.76 ± 0.24
 17 gi|21069067 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Asplenium jahandiezii 60 4 1 39.38/7.31 1.00 ± 0.26 0.29* ± 0.10 0.02* ± 0.01 0.09* ± 0.08
 18 gi|460379814 RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum 203 10 4 64.53/5.46 5.11* ± 0.34 2.87* ± 0.15 1.26 ± 0.06 2.24* ± 0.19
 19 gi|460366131 RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum 53 4 2 63.24/5.72 1.15 ± 0.36 2.81* ± 0.25 0.69 ± 0.08 0.28* ± 0.09
 20 gi|92087012 Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum 65 6 3 53.43/6.55 0.77 ± 0.12 10.02* ± 0.24 0.09* ± 0.01 0.01* ± 0.01
 21 gi|92087012 Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum 121 14 6 53.43/6.55 1.18 ± 0.07 32.57* ± 0.19 0.08* ± 0.01 0.01* ± 0.01
 22 gi|460375527 Ferredoxin–NADP reductase, leaf-type isozyme, Solanum lycopersicum 118 13 3 40.90/8.67 0.28* ± 0.07 0.93 ± 0.47 0.42* ± 0.10 0.14* ± 0.02
 23 gi|460373374 FerredoxinvNADP reductase, leaf-type isozyme, Solanum lycopersicum 299 20 5 40.77/8.37 0.82 ± 0.07 0.61 ± 0.07 0.48* ± 0.02 0.65 ± 0.05
 24 gi|350537679 Carbonic anhydrase, Solanum lycopersicum 278 17 4 34.85/6.67 1.45 ± 0.12 3.21* ± 0.27 0.85 ± 0.12 0.38* ± 0.05
 25 gi|350537679 Carbonic anhydrase, Solanum lycopersicum 98 5 1 34.85/6.67 0.65 ± 0.15 0.35* ± 0.03 1.53 ± 0.18 2.81* ± 0.23
 26 gi|460389468 Protein TIC 62, Solanum lycopersicum 313 13 5 52.96/7.66 1.02 ± 0.14 0.20* ± 0.11 0.13* ± 0.04 0.70 ± 0.17
 27 gi|460372959 Peptidyl-prolyl cis-trans isomerase CYP38, Solanum lycopersicum 185 17 5 49.54/5.00 0.92 ± 0.12 1.61 ± 0.38 0.26* ± 0.05 0.15* ± 0.04
Carbohydrate metabolism and energy
 28 gi|469517896 Glyceraldehyde-3-phosphate dehydrogenase, Atropa belladonna 101 10 4 34.16/6.04 0.88 ± 0.11 0.98 ± 0.29 0.46* ± 0.03 0.44* ± 0.13
 29 gi|460414390 ATP synthase delta chain, Solanum lycopersicum 166 6 4 27.32/8.90 0.49* ± 0.17 1.62 ± 0.23 0.38* ± 0.05 0.11* ± 0.03
 30 gi|303279681 Aryl-al/cohol dehydrogenase related protein, Micromonas pusilla 54 3 1 41.07/8.65 0.53 ± 0.11 1.05 ± 0.21 2.49* ± 0.35 1.28 ± 0.19
 31 gi|460397188 Thiamine thiazole synthase, Solanum lycopersicum 142 14 4 37.68/5.42 0.30* ± 0.21 3.06* ± 0.09 2.25* ± 0.07 0.23* ± 0.17
 32 gi|460365268 Biotin carboxylase 1, Solanum lycopersicum 413 13 6 59.08/6.52 0.68 ± 0.27 1.51 ± 0.26 0.82 ± 0.08 0.37* ± 0.13
 33 gi|723747143 Aconitate hydratase, Solanum lycopersicum 218 5 4 107.83/6.52 67.86* ± 0.19 3.13* ± 0.22 0.26* ± 0.02 5.77* ± 0.85
 34 gi|350538543 Succinic semialdehyde reductase isofom 2, Solanum lycopersicum 123 7 2 38.94/8.50 2.01* ± 0.26 2.52* ± 0.10 1.24 ± 0.09 1.06 ± 0.93
 35 gi|460408278 Enolase-like, Solanum lycopersicum 207 11 4 48.21/5.99 3.37* ± 0.29 4.38* ± 0.04 1.49 ± 0.09 1.14 ± 0.14
 36 gi|460415839 Probable ATP synthase 24 kDa subunit, Solanum lycopersicum 78 5 1 27.75/8.69 0.44* ± 0.08 0.49* ± 0.12 1.31 ± 0.09 1.02 ± 0.28
 37 gi|460373820 ATP synthase gamma chain, Solanum lycopersicum 167 12 3 41.75/8.15 1.93 ± 0.33 3.80* ± 0.24 0.09* ± 0.01 0.05* ± 0.01
 38 gi|460365435 Isocitrate dehydrogenase [NADP], Solanum lycopersicum 172 12 4 47.00/6.35 1.05 ± 0.22 0.27* ± 0.12 0.21* ± 0.03 0.86 ± 0.14
 39 gi|350535679 Cytosolic NADP-malic enzyme, Solanum lycopersicum 167 6 3 64.63/5.71 2.04* ± 0.20 1.20 ± 0.14 0.48* ± 0.07 0.49* ± 0.20
 40 gi|31088232 Chitinase, Solanum lycopersicum 123 18 7 28.05/5.93 0.82 ± 0.11 0.30* ± 0.03 1.51 ± 0.31 4.12* ± 0.10
Proteometabolism
 41 gi|350535160 Wound-inducible carboxypeptidase precursor, Solanum lycopersicum 191 5 2 56.04/5.84 1.14 ± 0.07 0.78 ± 0.05 3.62* ± 0.30 5.27* ± 0.22
 42 gi|350535160 Wound-inducible carboxypeptidase precursor, Solanum lycopersicum 177 5 3 56.04/5.84 1.07 ± 0.17 0.48* ± 0.02 2.72* ± 0.19 6.05* ± 0.30
 43 gi|350534564 26S protease regulatory subunit 6A homolog, Solanum lycopersicum 253 17 6 47.70/4.94 1.18 ± 0.05 0.78 ± 0.25 0.20* ± 0.07 0.30* ± 0.04
 44 gi|460393754 26S protease regulatory subunit 6B homolog, Solanum lycopersicum 198 7 2 46.80/5.63 1.24 ± 0.40 0.38* ± 0.13 0.33* ± 0.23 0.91 ± 0.34
 45 gi|460400419 Elongation factor G, Solanum lycopersicum 258 11 5 86.79/5.45 3.75* ± 0.31 0.53 ± 0.02 0.47* ± 0.01 3.29* ± 0.39
 46 gi|460415494 Eukaryotic initiation factor 4A-2, Solanum lycopersicum 370 18 7 47.10/5.46 1.82 ± 0.06 1.72 ± 0.15 0.35* ± 0.02 0.37* ± 0.02
 47 gi|460399092 Eukaryotic initiation factor 4A-2, Solanum lycopersicum 304 13 4 47.14/5.54 1.22 ± 0.22 2.73* ± 0.07 0.49* ± 0.22 0.22* ± 0.05
 48 gi|460391817 Elongation factor TuB, Solanum lycopersicum 125 5 2 56.29/6.69 0.62 ± 0.16 4.33* ± 0.23 2.45* ± 0.23 0.34* ± 0.03
 49 gi|460399098 Elongation factor 2 isoform X1, Solanum lycopersicum 248 7 4 94.97/5.84 1.35 ± 0.24 3.39* ± 0.23 3.06* ± 0.11 1.22 ± 0.19
 50 gi|460396224 Eukaryotic translation initiation factor 3 subunit K, Solanum lycopersicum 134 11 2 26.55/5.28 0.46* ± 0.03 1.12 ± 0.17 2.80* ± 0.26 1.15 ± 0.12
 51 gi|460391351 Cell division cycle protein 48 homolog, Solanum lycopersicum 172 9 5 90.15/5.10 24.39* ± 0.39 1.57 ± 0.13 0.38* ± 0.04 5.90* ± 0.81
 52 gi|460411520 Cell division cycle protein 48 homolog, Solanum lycopersicum 138 5 3 90.25/5.20 5.85* ± 0.22 0.91 ± 0.08 1.09 ± 0.04 7.00* ± 0.60
 53 gi|2492530 Leucine aminopeptidase 2, Solanum lycopersicum 79 8 3 60.08/8.18 4.80* ± 0.15 2.68* ± 0.29 0.81 ± 0.07 1.46 ± 0.07
 54 gi|350536267 Subtilisin-like protease precursor, Solanum lycopersicum 301 5 3 79.57/6.25 0.24* ± 0.02 0.07* ± 0.01 4.17* ± 0.29 14.46* ± 0.40
 55 gi|460381101 28 kDa ribonucleoprotein, Solanum lycopersicum 100 8 3 32.69/4.70 1.11 ± 0.19 1.15 ± 0.31 2.17* ± 0.70 2.05* ± 0.25
Amino acid metabolism
 56 gi|723717714 Phosphoglycerate kinase, Solanum lycopersicum 275 17 6 42.26/5.78 1.24 ± 0.46 0.47* ± 0.02 0.42* ± 0.02 1.11 ± 0.41
 57 gi|460404838 Adenosylhomocysteinase, Solanum lycopersicum 119 12 4 53.59/5.57 1.10 ± 0.34 0.53 ± 0.16 0.21* ± 0.06 0.45* ± 0.19
 58 gi|460395681 Ketol-acid reductoisomerase, Solanum lycopersicum 68 5 2 64.19/6.31 0.83 ± 0.40 0.34* ± 0.20 0.39* ± 0.09 0.97 ± 0.21
 59 gi|460415192 S-adenosylmethionine synthase 2, Solanum lycopersicum 207 7 2 43.51/5.41 2.09* ± 0.26 2.04* ± 0.07 0.64 ± 0.06 0.86 ± 0.09
 60 gi|460399143 Glycine dehydrogenase (decarboxylating), Solanum lycopersicum 192 9 6 114.02/6.69 1.90 ± 0.14 1.39 ± 0.08 4.02* ± 0.25 5.51* ± 0.19
 61 gi|460399143 Glycine dehydrogenase (decarboxylating), Solanum lycopersicum 153 7 5 114.02/6.69 3.80* ± 0.19 5.08* ± 0.31 6.81* ± 0.38 5.09* ± 0.21
 62 gi|460370413 Glycine dehydrogenase, Solanum lycopersicum 183 9 3 42.52/6.56 0.28* ± 0.02 12.57* ± 0.28 3.33* ± 0.16 0.07* ± 0.02
 63 gi|170458 Threonine deaminase, partial, Solanum lycopersicum 194 9 4 65.05/5.26 1.01 ± 0.01 0.82 ± 0.04 5.90* ± 0.09 7.29* ± 0.29
 64 gi|170458 Threonine deaminase, partial, Solanum lycopersicum 443 18 7 65.05/5.26 0.22* ± 0.01 0.40* ± 0.02 5.28* ± 0.62 2.96* ± 0.28
 65 gi|170458 Threonine deaminase, partial, Solanum lycopersicum 175 7 3 65.05/5.26 0.97 ± 0.01 0.55 ± 0.02 4.35* ± 0.03 7.62* ± 0.14
 66 gi|460398434 Cysteine synthase, Solanum lycopersicum 317 20 4 41.26/5.41 0.99 ± 0.02 6.39* ± 0.21 2.11* ± 0.22 0.33* ± 0.04
 67 gi|460404180 Cysteine synthase, Solanum lycopersicum 274 17 5 34.34/5.93 1.89 ± 0.13 0.63 ± 0.38 0.33* ± 0.16 1.09 ± 0.19
Chaperones
 68 gi|460389504 Protein disulfide-isomerase-like, Solanum lycopersicum 82 6 2 55.11/4.81 0.53 ± 0.02 0.55 ± 0.01 3.51* ± 0.28 3.39* ± 0.22
 69 gi|460395973 Heat shock protein 83, Solanum lycopersicum 76 1 1 90.77/5.23 0.96 ± 0.04 1.99 ± 0.03 19.73* ± 0.24 9.53* ± 0.23
 70 gi|460395973 Heat shock protein 83, Solanum lycopersicum 193 3 3 90.77/5.23 1.24 ± 0.23 3.10* ± 0.37 2.07* ± 0.26 0.82 ± 0.07
 71 gi|762844 Hsc70, Solanum lycopersicum 115 4 2 71.87/5.18 5.38* ± 0.40 0.60 ± 0.15 1.04 ± 0.28 9.20* ± 0.21
 72 gi|170386 Glucose-regulated protein 78, Solanum lycopersicum 211 12 3 41.32/8.51 2.45* ± 0.40 0.73 ± 0.14 1.31 ± 0.26 4.32* ± 0.41
 73 gi|145341034 AAA-metalloprotease FtsH, Ostreococcus lucimarinus 131 6 4 67.77/5.23 0.92 ± 0.05 0.63 ± 0.29 0.44* ± 0.01 0.75 ± 0.32
Signal transduction
 74 gi|1168191 14-3-3 protein 4 OS, Solanum lycopersicum 58 25 4 29.40/4.69 0.78 ± 0.33 0.67 ± 0.29 0.10* ± 0.04 0.11* ± 0.05
 75 gi|3041662 14-3-3 protein 3, Solanum lycopersicum 165 12 2 29.40/4.74 0.56 ± 0.23 1.33 ± 0.24 0.21* ± 0.04 0.08* ± 0.02
 76 gi|460405902 Plasma membrane-associated cation-binding protein 1, Solanum lycopersicum 95 6 1 21.98/5.03 1.71 ± 0.37 0.99 ± 0.19 0.20* ± 0.04 0.34* ± 0.04
 77 gi|590715109 Transducin family protein/WD-40 repeat family protein isoform 1, Theobroma cacao 57 2 1 87.16/6.40 0.82 ± 0.01 0.18* ± 0.01 2.87* ± 0.03 12.76* ± 0.11
 78 gi|460393840 Proliferation-associated protein 2G4-like, Solanum lycopersicum 134 7 2 43.07/6.41 2.16* ± 0.17 0.59 ± 0.15 0.27* ± 0.09 0.97 ± 0.19
 79 gi|525314284 Hop-interacting protein THI113, Solanum lycopersicum 117 10 2 37.34/5.82 0.93 ± 0.14 0.11* ± 0.07 0.24* ± 0.18 1.96 ± 0.10
Detoxification and antioxidation
 80 gi|350536897 Cytosolic ascorbate peroxidase 1, Solanum lycopersicum 113 14 2 27.73/5.61 1.54 ± 0.26 0.66 ± 0.06 0.42* ± 0.09 0.96 ± 0.09
 81 gi|350536897 Cytosolic ascorbate peroxidase 1, Solanum lycopersicum 115 7 1 27.73/5.61 7.20* ± 0.13 7.42* ± 0.32 6.03* ± 0.16 5.85* ± 0.07
 82 gi|350539113 Ascorbate peroxidase, Solanum lycopersicum 141 5 2 42.38/8.65 1.90 ± 0.20 0.75 ± 0.18 0.32* ± 0.04 0.81 ± 0.07
 83 gi|460373807 Putative lactoylglutathione lyase, Solanum lycopersicum 126 15 5 32.95/5.95 0.82 ± 0.22 0.17* ± 0.07 0.44* ± 0.21 2.05* ± 0.24
 84 gi|50400860 Monodehydroascorbate reductase, Solanum lycopersicum 255 17 5 47.12/5.77 0.85 ± 0.07 0.49* ± 0.17 0.40* ± 0.18 0.69 ± 0.16
 85 gi|251895 Polyphenol oxidase, Solanum lycopersicum 169 7 5 66.83/6.61 1.96 ± 0.06 2.87* ± 0.04 15.32* ± 0.10 10.49* ± 0.42
 86 gi|460397526 Heme-binding protein 2, Solanum lycopersicum 183 17 3 26.12/7.59 0.84 ± 0.08 0.82 ± 0.05 4.29* ± 0.25 4.41* ± 0.18
  1. aNumbering corresponds to the 2-DE in Fig. 3
  2. bAccession number from the NCBI nr database
  3. cNames and species of the proteins obtained via the MASCOT software from the NCBI nr database
  4. dMolecular weight search (MOWSE) score probability for the entire protein identified by the MASCOT software
  5. eThe sequence coverage of identified proteins
  6. fTotal numbers of identified peptides
  7. gMolecular weight and isoelectric point of the identified proteins
  8. hProtein abundance ratio of Treatment/Control tomato cultivars, with each value representing the mean value ± SD of three biological replicates
  9. Indicates significant (more than 2.0-fold or less than 0.50-fold) difference between control and treatment tomato cultivars