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Table 1 A total of 86 differentially expressed proteins identified in resistant and susceptible tomato cultivars after TYLCV infection

From: Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

aSpot no.

bAccession no.

cProtein name, Orgnaism

dProtein score

eSC

fMP

gMW (kDa) /pI

hZC/JC

hZT/JT

hZT/ZC

hJT/JC

Photosynthesis

 1

gi|407970998

Chlorophyll a-b binding protein 4, Solanum lycopersicum

135

13

2

28.98/5.33

0.31* ± 0.15

0.45* ± 0.35

0.22* ± 0.13

0.18* ± 0.09

 2

gi|115813

Chlorophyll a-b binding protein 8, Solanum lycopersicum

212

15

5

29.34/8.96

1.09 ± 0.22

0.45* ± 0.35

0.25* ± 0.06

0.90 ± 0.33

 3

gi|460405507

Chlorophyll a-b binding protein 8, Solanum lycopersicum

81

4

1

29.26/8.65

1.34 ± 0.25

1.78 ± 0.30

0.46* ± 0.03

0.36* ± 0.13

 4

gi|460375240

Oxygen-evolving enhancer protein 1, Solanum lycopersicum

273

10

2

35.15/5.91

0.84 ± 0.07

0.18* ± 0.01

0.31* ± 0.04

1.49 ± 0.38

 5

gi|460372520

Oxygen-evolving enhancer protein 1, Solanum lycopersicum

99

19

2

35.48/5.84

1.93 ± 0.19

0.18* ± 0.01

2.21* ± 0.44

10.57* ± 0.52

 6

gi|460408969

Rubisco accumulation factor 1, Solanum lycopersicum

119

9

3

50.46/5.10

5.36* ± 0.28

1.85 ± 0.28

0.77 ± 0,29

2.18* ± 0.39

 7

gi|460401823

Ribulose bisphosphate carboxylase/oxygenase activase 1, Solanum lycopersicum

56

3

1

49.05/8.15

0.09* ± 0.04

0.57 ± 0.12

6.75* ± 0.18

1.07 ± 0.29

 8

gi|723739979

Ribulose bisphosphate carboxylase/oxygenase activase, Solanum lycopersicum

285

11

3

50.97/8.76

0.88 ± 0.20

0.38* ± 0.30

0.07* ± 0.05

0.16* ± 0.01

 9

gi|1778414

Ribulose 1,5-bisphosphate carboxylase/oxygenase activase, Oryza sativa

126

7

2

48.06/5.85

0.39* ± 0.04

0.78 ± 0.16

3.45* ± 0.40

1.73 ± 0.01

 10

gi|100380

Ribulose bisphosphate carboxylase activase, Nicotiana tabacum

103

7

1

26.08/5.01

0.05* ± 0.01

0.03* ± 0.02

3.25* ± 0.44

5.57* ± 0.43

 11

gi|488453358

Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum

171

6

3

48.57/6.71

1.22 ± 0.14

43.48* ± 0.38

0.04* ± 0.01

0.01* ± 0,01

 12

gi|488453358

Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum

313

13

5

48.57/6.71

1.58 ± 0.11

23.71* ± 0.28

0.06* ± 0.01

0.02* ± 0.01

13

gi|488453358

Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum

62

4

2

48.57/6.71

1.37 ± 0.22

4.53* ± 0.25

0.05* ± 0.01

0.05* ± 0.01

 14

gi|488453358

Ribulose 1,5-bisphosphate carboxylase, Solanum lycopersicum

124

8

3

48.57/6.71

0.83 ± 0.15

1.89 ± 0.20

0.09* ± 0.01

0.04* ± 0.01

 15

gi|1293000

Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Cheirodendron trigynum

200

6

3

52.90/6.31

0.63 ± 0.04

1.18 ± 0.20

0.10* ± 0.05

0.06* ± 0.01

 16

gi|168282

Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Lactoris fernandeziana

281

16

4

44.16/6.33

0.19* ± 0.02

0.28* ± 0.02

2.60* ± 0.39

1.76 ± 0.24

 17

gi|21069067

Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit, Asplenium jahandiezii

60

4

1

39.38/7.31

1.00 ± 0.26

0.29* ± 0.10

0.02* ± 0.01

0.09* ± 0.08

 18

gi|460379814

RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum

203

10

4

64.53/5.46

5.11* ± 0.34

2.87* ± 0.15

1.26 ± 0.06

2.24* ± 0.19

 19

gi|460366131

RubisCO large subunit-binding protein subunit beta, Solanum lycopersicum

53

4

2

63.24/5.72

1.15 ± 0.36

2.81* ± 0.25

0.69 ± 0.08

0.28* ± 0.09

 20

gi|92087012

Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum

65

6

3

53.43/6.55

0.77 ± 0.12

10.02* ± 0.24

0.09* ± 0.01

0.01* ± 0.01

 21

gi|92087012

Ribulose bisphosphate carboxylase large chain OS, Solanum lycopersicum

121

14

6

53.43/6.55

1.18 ± 0.07

32.57* ± 0.19

0.08* ± 0.01

0.01* ± 0.01

 22

gi|460375527

Ferredoxin–NADP reductase, leaf-type isozyme, Solanum lycopersicum

118

13

3

40.90/8.67

0.28* ± 0.07

0.93 ± 0.47

0.42* ± 0.10

0.14* ± 0.02

 23

gi|460373374

FerredoxinvNADP reductase, leaf-type isozyme, Solanum lycopersicum

299

20

5

40.77/8.37

0.82 ± 0.07

0.61 ± 0.07

0.48* ± 0.02

0.65 ± 0.05

 24

gi|350537679

Carbonic anhydrase, Solanum lycopersicum

278

17

4

34.85/6.67

1.45 ± 0.12

3.21* ± 0.27

0.85 ± 0.12

0.38* ± 0.05

 25

gi|350537679

Carbonic anhydrase, Solanum lycopersicum

98

5

1

34.85/6.67

0.65 ± 0.15

0.35* ± 0.03

1.53 ± 0.18

2.81* ± 0.23

 26

gi|460389468

Protein TIC 62, Solanum lycopersicum

313

13

5

52.96/7.66

1.02 ± 0.14

0.20* ± 0.11

0.13* ± 0.04

0.70 ± 0.17

 27

gi|460372959

Peptidyl-prolyl cis-trans isomerase CYP38, Solanum lycopersicum

185

17

5

49.54/5.00

0.92 ± 0.12

1.61 ± 0.38

0.26* ± 0.05

0.15* ± 0.04

Carbohydrate metabolism and energy

 28

gi|469517896

Glyceraldehyde-3-phosphate dehydrogenase, Atropa belladonna

101

10

4

34.16/6.04

0.88 ± 0.11

0.98 ± 0.29

0.46* ± 0.03

0.44* ± 0.13

 29

gi|460414390

ATP synthase delta chain, Solanum lycopersicum

166

6

4

27.32/8.90

0.49* ± 0.17

1.62 ± 0.23

0.38* ± 0.05

0.11* ± 0.03

 30

gi|303279681

Aryl-al/cohol dehydrogenase related protein, Micromonas pusilla

54

3

1

41.07/8.65

0.53 ± 0.11

1.05 ± 0.21

2.49* ± 0.35

1.28 ± 0.19

 31

gi|460397188

Thiamine thiazole synthase, Solanum lycopersicum

142

14

4

37.68/5.42

0.30* ± 0.21

3.06* ± 0.09

2.25* ± 0.07

0.23* ± 0.17

 32

gi|460365268

Biotin carboxylase 1, Solanum lycopersicum

413

13

6

59.08/6.52

0.68 ± 0.27

1.51 ± 0.26

0.82 ± 0.08

0.37* ± 0.13

 33

gi|723747143

Aconitate hydratase, Solanum lycopersicum

218

5

4

107.83/6.52

67.86* ± 0.19

3.13* ± 0.22

0.26* ± 0.02

5.77* ± 0.85

 34

gi|350538543

Succinic semialdehyde reductase isofom 2, Solanum lycopersicum

123

7

2

38.94/8.50

2.01* ± 0.26

2.52* ± 0.10

1.24 ± 0.09

1.06 ± 0.93

 35

gi|460408278

Enolase-like, Solanum lycopersicum

207

11

4

48.21/5.99

3.37* ± 0.29

4.38* ± 0.04

1.49 ± 0.09

1.14 ± 0.14

 36

gi|460415839

Probable ATP synthase 24 kDa subunit, Solanum lycopersicum

78

5

1

27.75/8.69

0.44* ± 0.08

0.49* ± 0.12

1.31 ± 0.09

1.02 ± 0.28

 37

gi|460373820

ATP synthase gamma chain, Solanum lycopersicum

167

12

3

41.75/8.15

1.93 ± 0.33

3.80* ± 0.24

0.09* ± 0.01

0.05* ± 0.01

 38

gi|460365435

Isocitrate dehydrogenase [NADP], Solanum lycopersicum

172

12

4

47.00/6.35

1.05 ± 0.22

0.27* ± 0.12

0.21* ± 0.03

0.86 ± 0.14

 39

gi|350535679

Cytosolic NADP-malic enzyme, Solanum lycopersicum

167

6

3

64.63/5.71

2.04* ± 0.20

1.20 ± 0.14

0.48* ± 0.07

0.49* ± 0.20

 40

gi|31088232

Chitinase, Solanum lycopersicum

123

18

7

28.05/5.93

0.82 ± 0.11

0.30* ± 0.03

1.51 ± 0.31

4.12* ± 0.10

Proteometabolism

 41

gi|350535160

Wound-inducible carboxypeptidase precursor, Solanum lycopersicum

191

5

2

56.04/5.84

1.14 ± 0.07

0.78 ± 0.05

3.62* ± 0.30

5.27* ± 0.22

 42

gi|350535160

Wound-inducible carboxypeptidase precursor, Solanum lycopersicum

177

5

3

56.04/5.84

1.07 ± 0.17

0.48* ± 0.02

2.72* ± 0.19

6.05* ± 0.30

 43

gi|350534564

26S protease regulatory subunit 6A homolog, Solanum lycopersicum

253

17

6

47.70/4.94

1.18 ± 0.05

0.78 ± 0.25

0.20* ± 0.07

0.30* ± 0.04

 44

gi|460393754

26S protease regulatory subunit 6B homolog, Solanum lycopersicum

198

7

2

46.80/5.63

1.24 ± 0.40

0.38* ± 0.13

0.33* ± 0.23

0.91 ± 0.34

 45

gi|460400419

Elongation factor G, Solanum lycopersicum

258

11

5

86.79/5.45

3.75* ± 0.31

0.53 ± 0.02

0.47* ± 0.01

3.29* ± 0.39

 46

gi|460415494

Eukaryotic initiation factor 4A-2, Solanum lycopersicum

370

18

7

47.10/5.46

1.82 ± 0.06

1.72 ± 0.15

0.35* ± 0.02

0.37* ± 0.02

 47

gi|460399092

Eukaryotic initiation factor 4A-2, Solanum lycopersicum

304

13

4

47.14/5.54

1.22 ± 0.22

2.73* ± 0.07

0.49* ± 0.22

0.22* ± 0.05

 48

gi|460391817

Elongation factor TuB, Solanum lycopersicum

125

5

2

56.29/6.69

0.62 ± 0.16

4.33* ± 0.23

2.45* ± 0.23

0.34* ± 0.03

 49

gi|460399098

Elongation factor 2 isoform X1, Solanum lycopersicum

248

7

4

94.97/5.84

1.35 ± 0.24

3.39* ± 0.23

3.06* ± 0.11

1.22 ± 0.19

 50

gi|460396224

Eukaryotic translation initiation factor 3 subunit K, Solanum lycopersicum

134

11

2

26.55/5.28

0.46* ± 0.03

1.12 ± 0.17

2.80* ± 0.26

1.15 ± 0.12

 51

gi|460391351

Cell division cycle protein 48 homolog, Solanum lycopersicum

172

9

5

90.15/5.10

24.39* ± 0.39

1.57 ± 0.13

0.38* ± 0.04

5.90* ± 0.81

 52

gi|460411520

Cell division cycle protein 48 homolog, Solanum lycopersicum

138

5

3

90.25/5.20

5.85* ± 0.22

0.91 ± 0.08

1.09 ± 0.04

7.00* ± 0.60

 53

gi|2492530

Leucine aminopeptidase 2, Solanum lycopersicum

79

8

3

60.08/8.18

4.80* ± 0.15

2.68* ± 0.29

0.81 ± 0.07

1.46 ± 0.07

 54

gi|350536267

Subtilisin-like protease precursor, Solanum lycopersicum

301

5

3

79.57/6.25

0.24* ± 0.02

0.07* ± 0.01

4.17* ± 0.29

14.46* ± 0.40

 55

gi|460381101

28 kDa ribonucleoprotein, Solanum lycopersicum

100

8

3

32.69/4.70

1.11 ± 0.19

1.15 ± 0.31

2.17* ± 0.70

2.05* ± 0.25

Amino acid metabolism

 56

gi|723717714

Phosphoglycerate kinase, Solanum lycopersicum

275

17

6

42.26/5.78

1.24 ± 0.46

0.47* ± 0.02

0.42* ± 0.02

1.11 ± 0.41

 57

gi|460404838

Adenosylhomocysteinase, Solanum lycopersicum

119

12

4

53.59/5.57

1.10 ± 0.34

0.53 ± 0.16

0.21* ± 0.06

0.45* ± 0.19

 58

gi|460395681

Ketol-acid reductoisomerase, Solanum lycopersicum

68

5

2

64.19/6.31

0.83 ± 0.40

0.34* ± 0.20

0.39* ± 0.09

0.97 ± 0.21

 59

gi|460415192

S-adenosylmethionine synthase 2, Solanum lycopersicum

207

7

2

43.51/5.41

2.09* ± 0.26

2.04* ± 0.07

0.64 ± 0.06

0.86 ± 0.09

 60

gi|460399143

Glycine dehydrogenase (decarboxylating), Solanum lycopersicum

192

9

6

114.02/6.69

1.90 ± 0.14

1.39 ± 0.08

4.02* ± 0.25

5.51* ± 0.19

 61

gi|460399143

Glycine dehydrogenase (decarboxylating), Solanum lycopersicum

153

7

5

114.02/6.69

3.80* ± 0.19

5.08* ± 0.31

6.81* ± 0.38

5.09* ± 0.21

 62

gi|460370413

Glycine dehydrogenase, Solanum lycopersicum

183

9

3

42.52/6.56

0.28* ± 0.02

12.57* ± 0.28

3.33* ± 0.16

0.07* ± 0.02

 63

gi|170458

Threonine deaminase, partial, Solanum lycopersicum

194

9

4

65.05/5.26

1.01 ± 0.01

0.82 ± 0.04

5.90* ± 0.09

7.29* ± 0.29

 64

gi|170458

Threonine deaminase, partial, Solanum lycopersicum

443

18

7

65.05/5.26

0.22* ± 0.01

0.40* ± 0.02

5.28* ± 0.62

2.96* ± 0.28

 65

gi|170458

Threonine deaminase, partial, Solanum lycopersicum

175

7

3

65.05/5.26

0.97 ± 0.01

0.55 ± 0.02

4.35* ± 0.03

7.62* ± 0.14

 66

gi|460398434

Cysteine synthase, Solanum lycopersicum

317

20

4

41.26/5.41

0.99 ± 0.02

6.39* ± 0.21

2.11* ± 0.22

0.33* ± 0.04

 67

gi|460404180

Cysteine synthase, Solanum lycopersicum

274

17

5

34.34/5.93

1.89 ± 0.13

0.63 ± 0.38

0.33* ± 0.16

1.09 ± 0.19

Chaperones

 68

gi|460389504

Protein disulfide-isomerase-like, Solanum lycopersicum

82

6

2

55.11/4.81

0.53 ± 0.02

0.55 ± 0.01

3.51* ± 0.28

3.39* ± 0.22

 69

gi|460395973

Heat shock protein 83, Solanum lycopersicum

76

1

1

90.77/5.23

0.96 ± 0.04

1.99 ± 0.03

19.73* ± 0.24

9.53* ± 0.23

 70

gi|460395973

Heat shock protein 83, Solanum lycopersicum

193

3

3

90.77/5.23

1.24 ± 0.23

3.10* ± 0.37

2.07* ± 0.26

0.82 ± 0.07

 71

gi|762844

Hsc70, Solanum lycopersicum

115

4

2

71.87/5.18

5.38* ± 0.40

0.60 ± 0.15

1.04 ± 0.28

9.20* ± 0.21

 72

gi|170386

Glucose-regulated protein 78, Solanum lycopersicum

211

12

3

41.32/8.51

2.45* ± 0.40

0.73 ± 0.14

1.31 ± 0.26

4.32* ± 0.41

 73

gi|145341034

AAA-metalloprotease FtsH, Ostreococcus lucimarinus

131

6

4

67.77/5.23

0.92 ± 0.05

0.63 ± 0.29

0.44* ± 0.01

0.75 ± 0.32

Signal transduction

 74

gi|1168191

14-3-3 protein 4 OS, Solanum lycopersicum

58

25

4

29.40/4.69

0.78 ± 0.33

0.67 ± 0.29

0.10* ± 0.04

0.11* ± 0.05

 75

gi|3041662

14-3-3 protein 3, Solanum lycopersicum

165

12

2

29.40/4.74

0.56 ± 0.23

1.33 ± 0.24

0.21* ± 0.04

0.08* ± 0.02

 76

gi|460405902

Plasma membrane-associated cation-binding protein 1, Solanum lycopersicum

95

6

1

21.98/5.03

1.71 ± 0.37

0.99 ± 0.19

0.20* ± 0.04

0.34* ± 0.04

 77

gi|590715109

Transducin family protein/WD-40 repeat family protein isoform 1, Theobroma cacao

57

2

1

87.16/6.40

0.82 ± 0.01

0.18* ± 0.01

2.87* ± 0.03

12.76* ± 0.11

 78

gi|460393840

Proliferation-associated protein 2G4-like, Solanum lycopersicum

134

7

2

43.07/6.41

2.16* ± 0.17

0.59 ± 0.15

0.27* ± 0.09

0.97 ± 0.19

 79

gi|525314284

Hop-interacting protein THI113, Solanum lycopersicum

117

10

2

37.34/5.82

0.93 ± 0.14

0.11* ± 0.07

0.24* ± 0.18

1.96 ± 0.10

Detoxification and antioxidation

 80

gi|350536897

Cytosolic ascorbate peroxidase 1, Solanum lycopersicum

113

14

2

27.73/5.61

1.54 ± 0.26

0.66 ± 0.06

0.42* ± 0.09

0.96 ± 0.09

 81

gi|350536897

Cytosolic ascorbate peroxidase 1, Solanum lycopersicum

115

7

1

27.73/5.61

7.20* ± 0.13

7.42* ± 0.32

6.03* ± 0.16

5.85* ± 0.07

 82

gi|350539113

Ascorbate peroxidase, Solanum lycopersicum

141

5

2

42.38/8.65

1.90 ± 0.20

0.75 ± 0.18

0.32* ± 0.04

0.81 ± 0.07

 83

gi|460373807

Putative lactoylglutathione lyase, Solanum lycopersicum

126

15

5

32.95/5.95

0.82 ± 0.22

0.17* ± 0.07

0.44* ± 0.21

2.05* ± 0.24

 84

gi|50400860

Monodehydroascorbate reductase, Solanum lycopersicum

255

17

5

47.12/5.77

0.85 ± 0.07

0.49* ± 0.17

0.40* ± 0.18

0.69 ± 0.16

 85

gi|251895

Polyphenol oxidase, Solanum lycopersicum

169

7

5

66.83/6.61

1.96 ± 0.06

2.87* ± 0.04

15.32* ± 0.10

10.49* ± 0.42

 86

gi|460397526

Heme-binding protein 2, Solanum lycopersicum

183

17

3

26.12/7.59

0.84 ± 0.08

0.82 ± 0.05

4.29* ± 0.25

4.41* ± 0.18

  1. aNumbering corresponds to the 2-DE in Fig. 3
  2. bAccession number from the NCBI nr database
  3. cNames and species of the proteins obtained via the MASCOT software from the NCBI nr database
  4. dMolecular weight search (MOWSE) score probability for the entire protein identified by the MASCOT software
  5. eThe sequence coverage of identified proteins
  6. fTotal numbers of identified peptides
  7. gMolecular weight and isoelectric point of the identified proteins
  8. hProtein abundance ratio of Treatment/Control tomato cultivars, with each value representing the mean value ± SD of three biological replicates
  9. ∗Indicates significant (more than 2.0-fold or less than 0.50-fold) difference between control and treatment tomato cultivars