Fig. 1From: Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinaseAnalysis of interactions of selected members of the SnRK2 family with clade A PP2C phosphatases. a Yeast two-hybrid analysis; growth of yeast expressing the indicated constructs was monitored on selective media: without Leu and Trp (−LW); without Leu, Trp and His (−LWH) supplemented with different concentrations of aminotriazole (3-AT); without Leu, Trp and Ade (−LWA). AD, Gal4 activation domain; BD, Gal4 binding domain. Data represent one of three independent experiments showing similar results. b Pull down assays; GST-fused ABI1 or GST alone, bound to glutathione-sepharose beads, were incubated with recombinant SnRK2.4. Proteins bound to the resin were separated by SDS-PAGE, electroblotted to a membrane and visualized by immunoblotting using anti-SnRK2.4/SnRK2.10 antibodies. In parallel, visualization of proteins in samples used for the Western blotting was performed by staining with Coomassie Brilliant Blue, CBB. Data represent one of two independent experiments showing similar resultsBack to article page