Fig. 8

The effects of altered NtTTG2 protein quantities on subcellular localization of the NtARF8 protein and associated gene expression. a Confocal microscopic imaging of roots transformed with the ARF8-YFP fusion gene. Scale bars = 100 μm. b, c Percentage of root cells showing TTG2-RFP and ARF8-YFP fluorescence signals, respectively, only in the cytoplasm, only in the nucleus, or in both compartments. Cells in totally 10 fields of vision were counted for RFP and YFP fluorescence signals, respectively. Data shown are mean values ± SEM bars (n = 360–390 cells). d Western blots showing protein levels in cytoplasmic (cy) and nuclear (nu) fractions isolated from leaf cells. PEPC and histone H3 were used as cytosolic and nuclear markers, respectively. e ChIP PCR analyses to quantify levels of the affinity for NtARF8-YFP binding with the GH3 promoter. f Expression levels of related genes quantified by RT-qPCR as the transcript quantity ratios to EF1α. Data shown in e, f are mean values ± SEM bars (n = 3 experimental replicates)