Fig. 5From: Tobacco TTG2 regulates vegetative growth and seed production via the predominant role of ARF8 in cooperation with ARF17 and ARF19Concurrent NtARF8, NtARF17, and NtARF19 silencing manipulations and the subsequent effects on plant growth. a NtARF8, NtARF17, and NtARF19 silencing efficiencies. Gene silencing was performed under TTG2 + background to evaluate the combinational effects of NtARF8, NtARF17, and NtNAR19 on the role of NtTTG2 in the plant growth. Gene expression levels were determined by RT-qPCR as transcript quantity ratios to EF1α. Percent reductions of NtARF transcript quantities in the different gene silencing manipulations were quantified in relative to TTG2 + background. Gene expression levels under backgrounds of WT, TTG2 + only, and TTG2 + with a single NtARF gene silencing are shown for comparisons of plant growth changes. b Plant fresh weight alterations by NtTTG2 overexpression and NtARF silencing compared to WT background. Data shown in a, b are mean values ± SEM bars (n = 6 experimental replicates)Back to article page