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Fig. 5 | BMC Plant Biology

Fig. 5

From: Tobacco TTG2 regulates vegetative growth and seed production via the predominant role of ARF8 in cooperation with ARF17 and ARF19

Fig. 5

Concurrent NtARF8, NtARF17, and NtARF19 silencing manipulations and the subsequent effects on plant growth. a NtARF8, NtARF17, and NtARF19 silencing efficiencies. Gene silencing was performed under TTG2 + background to evaluate the combinational effects of NtARF8, NtARF17, and NtNAR19 on the role of NtTTG2 in the plant growth. Gene expression levels were determined by RT-qPCR as transcript quantity ratios to EF1α. Percent reductions of NtARF transcript quantities in the different gene silencing manipulations were quantified in relative to TTG2 + background. Gene expression levels under backgrounds of WT, TTG2 + only, and TTG2 + with a single NtARF gene silencing are shown for comparisons of plant growth changes. b Plant fresh weight alterations by NtTTG2 overexpression and NtARF silencing compared to WT background. Data shown in a, b are mean values ± SEM bars (n = 6 experimental replicates)

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