Fig. 2

VIGS manipulations of NtARF genes and gene silencing efficiencies. a Diagrams of the two-component gene-silencing constructs. b Technical scheme. The two gel panels represent one of six experimental repeats each containing nine transfected WT:RFP plants tested in comparison with WT:RFP. Top gel panel is the result of PCR analysis of genomic DNA, and lower gel panel is the result of RT-PCR analysis. Codes of transfected plants are arbitrary. c Relative levels of different NtARFs in leaves of plants indicated under the corresponding bar graphs. The expression level of each NtARF was quantified by RT-qPCR as the transcript quantity ratio of the NtARF gene to EF1α used as a reference gene based on its constitutive expression. Data shown are mean values ± SEM bars (n = 6 experimental replicates)