Fig. 1

The expression of NtTTG2 in response to synthetic auxin NAA. Plants were treated by spraying over tops with a surfactant solution in control or with an NAA solution containing the surfactant. Relative levels of NtTTG2 expression were quantified by RT-qPCR as the transcript quantity ratios to the constitutively expressed EF1α gene used as a reference. Data shown are mean values ± standard errors from mean (SEMs) of results obtained from three experimental replicates, i.e., the number of replicates (n) = 3. Inset is Northern blot hybridization performed at 60 h after plant treatment