Fig. 1
Tissue-specific expression of MfPIP2-7 and influence of cold, and abscisic acid (ABA) on MfPIP2-7 transcripts. Mature leaflets, stem, and lateral roots were detached from 2-month-old seedlings (a). Plants were exposed to 5 °C in a growth chamber for cold treatment (b). Detached leaves placed in 0.1 mM ABA solution or H2O as control for 12 h (c), or pretreated in 1 mM naproxen solution for 2 h, followed by 8 h of cold treatment at 5 °C, while those continuously placed in H2O under room temperature were used as non-stressed control (d). Relative expression levels were determined by qRT-PCR and normalized to actin expression. The same letter above a column indicates no significant difference by Duncan’s test at P < 0.05