Fig. 3

LeEIL-1 Expression patterns and visual inspection of WT, EV, Ei, and EO hairy root lines. (a) Transcript levels of LeEIL-1 in the randomly selected hairy root lines of WT, EV, Ei, and EO cultured in B5 medium under light at 26–28 °C for 15 days with constant shaking at 80 rpm. A representative example from two biological experiments was shown; data represent means ± SD (n = 3) and the bars with different capital letters indicate significant differences at P < 0.01 (Least Significant Difference); (b) Phenotypes of WT, EV, Ei, and EO hairy roots in conical flasks containing hormone-free B5 liquid medium for multiplication culture; (c) Visual inspection for the color changes of WT, EV, Ei, and EO hairy root lines in M9 for 6 days (20 ml medium/50 ml flask); (d) Dynamic expression pattern analysis of LeEIL-1 when hairy root lines were transferred from B5 into M9 medium. The values of Ei and EO lines are significantly different from those of WT or EV lines at the time points of 6 or 9 days, respectively (Least Significant Difference, P < 0.05); (e) Expression patterns of LeEIL-1 of the WT-1 during the dark/light transition, in which hairy roots were pre-cultured under light for 2 days and then transferred into darkness. The asterisk indicates that the mean value in the dark was significantly different from that under light at 3, 6 or 9 days time point, respectively (Student’s t-test, P < 0.05). The sunshine logo and light gray represent light period for B5 culture condition, and the moon logo and deep gray represent night period for M9 culture condition