Fig. 1

Induction, verification, and growth polymorphism observation of four different types of hairy root lines. (a) Structure and restriction maps of the transformation vectors pBI121-eGFP (EV), pBI121-LeEIL-1-RNAi (Ei), and pBI121-LeEIL-1-Overexpression (EO); (b) Growth polymorphism of the induced L. erythrorhizon hairy roots by using the method of pricking nodes with a needle. Differentiated callus, leaf (c), and regenerated shoots (d) from the Ei hairy root lines on the stock culture medium; (e) PCR analysis of the rolC gene in hairy roots. The untransformed L. erythrorhizon seedling was used as the negative control (CK)