Fig. 1

LHY-defective mutants exhibit early flowering under both LDs and SDs. a Isolation of an LHY-defective mutant (lhy-7). It was isolated from a pool of T-DNA insertional lines deposited in the Arabidopsis Biological Resource Center (Ohio State University, OH). bp, base pair. F and R, forward and reverse primers, respectively, used to examine the expression of LHY gene. b Lack of LHY gene expression in lhy-7 mutant. Gene expression was examined by RT-PCR. A tubulin gene (TUB) was used as control for constitutive expression. c Flowering phenotypes of lhy mutants under LDs. The previously reported lhy-20 mutant was also included in the assays [39]. Plants were grown until flowering in soil under LDs (16-h light and 8-h dark) (left panel). Rosette leaf numbers of 20 plants were averaged and statistically treated using Student t-test (*P < 0.01) for each plant genotype (right panel). Bars indicate standard error of the mean (SE). d Flowering phenotypes of lhy mutants under SDs. Plants were grown until flowering in soil under SDs (8-h light and 16-h dark) (left panel). Flowering times were measured as described in (c) (right panel)