Fig. 7

Validation of the relative expression levels of differential expression transcripts by quantitative RT-PCR (qRT-PCR) analysis. a Expression profiles of the selected DEGs in the salt-treated calli relative to the control calli, as determined by qRT-PCR(24 h: blue; 120 h:green) and RNA-seq(24 h:red; 120 h: purple). The x-axis indicated the annotation of the selected DEGs. The y-axis indicated the normalized expression level of the genes. A: Calmodulin 1; B:Calcium-binding protein CML37; C: Calcium-dependent protein kinase 10; D: Calcium-dependent protein kinase 13-like; E: Mitogen-activated protein kinase kinase kinase A; F: Mitogen-activated protein kinase kinase kinase 2; G: Mitogen-activated protein kinase kinase kinase 2; H: Auxin influx carrier; I: Auxin response factor 4; J: G-type lectin S-receptor-like serine/threonine-protein kinase; K: Cysteine-rich receptor-like protein kinase 25; L: LRR receptor-like serine/threonine-protein kinase FLS2; M: WRKY transcription factor 75; N: WRKY transcription factor 40; O:WRKY transcription factor 29; P: Ethylene-responsive transcription factor ERF(AP2/ERF); Q: MYB-related protein MYB4; R: MYB superfamily protein 1; S: Methyltransferase PMT15; T: Caffoyl-CoA-O-methyltransferase; U: Caffeic acid 3-O-methyltransferase; V: Chalcone synthase; W: Respiratory burst oxidase homolog protein A; X:Respiratory burst oxidase homolog protein B; Y: Respiratory burst oxidase homolog protein D; Z: Pathogenesis-related protein STH-2. The transcriptional level of the selected genes was performed by qRT-PCR with three biological replications and action was used as an internal reference. Error barsrepresent the standard deviations of qRT-PCR signals (n ≥ 3). b Correlation of the expression ratio of selected DEGs analyzed by qRT-PCR and RNA-seq