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Table 1 Results from DIGE analysis of control vs salt treated EBC extract

From: Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins

DIGE

       

Spot No.

t-testa

Av. Ratiob

Protein(s) in Spotc

GO locationd

GO biological processe

Uniprot #

Species

Signifcant

Unique

peptidesf

M.W./pIg

664

0.00013

2.48

V-ATPase A subunit

TP

transport

Q9AVU8

M. crystallinum

12

69/5.09

740-1

  

Nitrite reductase

CP

nitrogen metabolism

B9RYH9

R. communis

4

66/6.04

 

9.70E-05

2.61

       

740-2

  

Beta-hexosaminidase 1

V

carbohydrate

metabolism

A7WM73

A. thaliana

3

61/5.88

760

0.00031

2.93

V-ATPase B subunit

TP

transport

Q8GUB5

M. crystallinum

31

54/4.96

765

0.0002

2.68

NADP-dependent malic enzyme

Cyt

CAM

P37223

M. crystallinum

46

64/6.06

767

0.00062

2.30

NADP-dependent malic enzyme

Cyt

CAM

P37223

M. crystallinum

49

64/6.06

774

0.0033

1.67

NADP-dependent malic enzyme

Cyt

CAM

P37223

M. crystallinum

48

64/6.06

775

0.0071

1.58

NADP-dependent malic enzyme

Cyt

CAM

P37223

M. crystallinum

54

64/6.06

923

0.0016

−1.79

no proteins identified

      

1062

0.00053

−1.59

Alpha-1,4-glucan protein synthase

AP

cell wall metabolism

I1J637

G. max

16

42/5.52

1064

0.0014

2.00

no proteins identified

      

1065

0.0026

1.96

Beta-D-galactosidase

AP

cell wall metabolism

Q5CCQ1

P. pyrifolia

3

41/5.5

1071-1

  

2-phosphoglycerate hydrolase

Cyt

glycolysis

Q43130

M. crystallinum

12

48/5.62

 

0.001

2.00

       

1071-2

  

Alpha-1,4-glucan protein

Cyt

cell wall metabolism

I1J637

G. max

9

42/5.52

1095

0.0034

1.69

Malate dehydrogenase

Cyt

CAM

Q645N1

S. lycopersicum

4

36/8.87

1123-1

  

Malate dehydrogenase

Cyt

CAM/

Q645N1

S. lycopersicum

5

36/8.87

 

0.00049

2.13

       

1123-2

  

Proline iminopeptidase

Cyt

proteolysis

B9G1Q0

P. trichocarpa

4

37/8.96

1153

3.20E-07

6.20

V-ATPase E subunit

TP

transport

Q40272

M.crystallinum

26

26/6.52

1290

0.032

−1.43

no proteins identified

      

1307

0.0019

2.20

Ascorbate peroxidase

Cyt

stress response

C5J0H6

S. nigrum

5

18/4.83

1325

0.0082

−1.66

Fructose bisphosphate aldolase,

Cyt

glycolysis

O04975

M. crystallinum

8

38/6.49

1501

2.40E-07

−2.51

Alpha-galactosidase-like protein

AP

cell wall metabolism

D7LVE6

A. lyrata

3

48/4.79

1502

0.003

1.63

5-methyltetrahydropteroyltri-glutamate-homocysteine methyltransferase

Cyt

amino acid metabolism

P93263

M. crystallinum

62

85/5.9

1503

0.03

−1.4

Triosephosphate isomerase

Cyt

glycolysis

I3SN66

M. tribuloides

5

33/6.54

1508

4.40E-05

2.4

V-ATPase A subunit

TP

transport

Q9AVU8

M. crystallinum

54

69/5.09

  1. Data are from 4 independent biological experiments for each treatmentaStudent’s t-test p values are given as a measure of confidence for the ratio of each spot measured
  2. bAverage ratios of spot abundance of salt-treated samples relative to the untreated control represent data from four independent experiments
  3. cProtein names, dGO cellular location and eGO biological function annotations are taken from the Uniprot database recommended name and annotation
  4. f p < 0.05; Peptide sequences, % sequence coverage, best Mascot ion, charge and delta ion score as well as spectra charge states can be seen for all identified proteins in Additional file 5
  5. gTheoretical molecular weight and isoelectric point for each protein spot identified