Fig. 5
From: Comparative expression profiling reveals a role of the root apoplast in local phosphate response
Fe and pectin staining of roots after seedling transfer from + Pi agar to + Pi or –Pi medium for up to 2 days. a Perls/DAB Fe staining (left panels) and Ruthenium Red (RR) staining of non-methylesterified pectins (center and right panels). Shown are overview images of Perls/DAB and RR stained roots as well as detail images of the elongation/early differentiation zone. Scale bar 100 μm. b Hydroxylamine ferric chloride (HFC) staining of methylesterified pectins. Shown are overview images of the root (scale bar 100 μm) as well as detail images of the root meristem (scale bar 100 μm) and the stem cell niche (scale bar 20 μm). Differences in coloration between overview and detail result from the use of different imaging devices. Arrows point to the SCN and cortical cell layer in the transition zone, asterisks label the quiescent center cells in the SCN. c Details of the Perls/DAB, RR and HFC staining in Pi-depleted cortical cells of Col plants and stem cells of pdr2 plants. Scale bar 20 μm