Fig. 6

Expression of EIL2 in large flowered Campanula during flower development and following ethylene exposure. Campanula species are C. formanekiana (Cf) and C. medium (Cm). In a, c the floral developmental stages were; day before opening (0), 1-day old flower (1), 2-days old flower (2) and 4-days old flower (4). In b, d ethylene was supplied to sealed glass tanks at the following concentrations; 0.00 μL · L⁻¹ (0), 0.025 μL · L⁻¹ (0.025) and 0.050 μL · L⁻¹ (0.050) ethylene. Results from RT-qPCR are presented as relative expressions in (a) and (b). The corresponding results of EIL2 RT-PCR loaded on agarose gels are presented in (c) and (d). The values of 1-day flowers and 0 μL · L⁻¹ ethylene were set to the value 1 in (a) and (b), respectively. As reference gene Actin (ACT) from Campanula was used, these RT-PCR results are presented in Figs. 4a and 5a. RT-qPCR data were normalized to transcripts of the same ACT. Data are means ± SE. Values with same letters are not significantly different (P > 0.05)