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Fig. 10 | BMC Plant Biology

Fig. 10

From: A natural frameshift mutation in Campanula EIL2 correlates with ethylene insensitivity in flowers

Fig. 10

Detection of EIL2 in hybrids of C. formanekiana (Cf) and C. medium (Cm). The original PCR products (EIL2/eil2) are 499 bp or 492 bp in Cf and Cm, respectively. Upon digestion with the restriction enzyme NlaIII CfEIL2 produces DNA fragments of 362 bp, 122 bp and 16 bp (a). In contrast Cm containing the 7 bp deletion in eil2 produces DNA fragments of 476 bp and 16 bp (b). The 16 bp DNA fragments are not detected. NlaIII restriction analysis of 5 heterozygote Cf × Cm hybrids shows that all hybrids contain both Cf and Cm specific DNA fragments (cg, hybrids A–E). Hybrids were produced according to [57]

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