Table 1 Adaptor and PCR primer sequences
Primer name | 5′–3′ sequence | Usage |
|---|---|---|
F1 | ATGGGATCGGTGGCAAACAAC | overlap ORF fragment1 |
R1 | GCTTGTTTGCAGAAACTTGT | overlap ORF fragment1 |
F2 | ATTTCTAAATGGTGTGCAAGC | overlap ORF fragment2 |
R2 | TTAGGGAGCTCGGGGGAAAG | overlap ORF fragment2 |
Oligo d (T)18 | TTTTTTTTTTTTTTTTTT | Reverse transcription |
AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 5′ -RACE |
AUAP | GGCCACGCGTCGACTAGTAC | 5′ -RACE |
GSP5′-1 | CTCTAAGCAAATGTCTTG | 5′ -RACE |
GSP5′-2 | TCCTCCTCCGCCCTTAG | 5′ -RACE |
GSP5′-3 | GTTTGCCACCGATCCCAT | 5′ -RACE |
Adaptor J-T | CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTTTT | 3′ -RACE |
Adaptor J-R | CTGATCTAGAGGTACCGGATCC | 3′ -RACE |
GSP3'-1 | TCAGAAGGTTCTACGACAGTGAG | 3′ -RACE |
GSP3′-2 | CCAGACCCAAATGATACTCGTGA | 3′ -RACE |
GSP3′-3 | TACACTATCTTTCCCCCGAGCTC | 3′ -RACE |
Full-F | TGGTGGAGATGGGATCGGTGGCA | ORF amplifications |
Full-R | CTAGTAAATATATTATACAAGTC | ORF amplifications |
SOS1s-Sal-F | GCGTCGACATGGGATCGGTGGCAAACAACGTG | Functional complementation |
SOS1s-Not-R | TTGCGGCCGCGATTAGGGAGCTCGGGGGAAAG | Functional complementation |
Actin-F | AGCTTGCATATGTTGCTCTTGA | qPCR |
Actin-R | TTACCGTAAAGGTCCTTCCTGA | qPCR |
DL-F | TGGAGCTGAGGATGAACA | qPCR |
DL-R | CTACCGTACTTTCTATGAACAC | qPCR |
actin-F | GTGATGTCGATGTCCGTAA | qPCR |
actin-R | AGAAGCCAAGATAGAACCA | qPCR |
CmEF1α-F | TTTTGGTATCTGGTCCTGGAG | qPCR |
CmEF1α-R | CCATTCAAGCGACAGACTCA | qPCR |
AtAct2-F | TTCGTTTTGCGTTTTAGTCCC | RT-PCR |
AtAct2-R | GGGAACAAAAGGAATAAAGAGGC | RT-PCR |