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Table 8 Linkage analysis of RG-I from NbPAGR-silenced N. benthamiana plants and PAGR-overexpressing Arabidopsis lines

From: A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans

  VIGS Control NbPAGR-Silenced Col-0 35S::PAGR-YFP line 6 35S-PAGR-YFP line 9
T-Ara 1.1 ± 0.4 1.0 ± 0.2 1.9 ± 0.5 1.4 ± 0.2 2.3 ± 0.6
2-Rha 6.2 ± 0.5 8.9 ± 0.1* 8.5 ± 1.0 7.1 ± 1.8 9.6 ± 1.1
3-Ara 0.7 ± 0.1 0.7 ± 0.1 1.3 ± 0.1 1.3 ± 0.1 1.2 ± 0.1
T-Gal 4.9 ± 0.4 6.7 ± 0.2 6.3 ± 0.3 6.0 ± 0.7 5.8 ± 0.5
5-Ara 8.4 ± 0.5 9.2 ± 0.7 16.5 ± 0.7 16.5 ± 0.6 16.0 ± 0.7
2,4-Rha 7.9 ± 0.1 8.8 ± 0.4 7.4 ± 1.2 7.6 ± 1.1 6.8 ± 0.4
3-Gal 4.4 ± 0.1 4.5 ± 0.3 3.5 ± 0.1 4.6 ± 0.2* 4.7 ± 0.2*
3,5-Ara 1.2 ± 0.1 1.4 ± 0.1* 6.4 ± 0.2 6.4 ± 0.5 5.9 ± 0.5
4-Gal 47.9 ± 0.4 42.0 ± 1.6* 19.4 ± 0.4 16.0 ± 0.6* 14.7 ± 0.6*
2,5-Ara ND ND 6.6 ± 0.4 6.1 ± 0.5 5.5 ± 0.5
6-Gal 2.5 ± 0.1 3.0 ± 0.2* 4.6 ± 0.3 5.6 ± 0.1* 6.3 ± 0.1*
3,4-Hex 1.7 ± 0.2 1.4 ± 0.1 8.9 ± 0.5 9.3 ± 1.3 8.0 ± 1.0
2,4-Hex 0.7 ± 0.1 0.6 ± 0.1 ND ND ND
4,6-Hex 1.6 ± 0.1 1.6 ± 0.2 1.8 ± 0.1 1.5 ± 0.1 1.3 ± 0.1
3,6-Hex 10.6 ± 0.2 10.4 ± 0.4 6.8 ± 0.3 10.7 ± 0.2* 11.7 ± 1.1*
  1. Values are the molar percentage of total sugars detected. Data shown are averages ± standard deviation. Asterisks indicate significant difference from wild type or control plants (p < 0.01, t-test). (ND: not detected)