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Fig. 6 | BMC Plant Biology

Fig. 6

From: A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans

Fig. 6

Biochemical characterization of PAGR overexpressing Arabidopsis lines. a The monosaccharide composition of cell walls from 35S::PAGR-YFP seedlings grown in liquid culture. Over-expressing lines exhibit an increase in total arabinose content and slight reductions in fucose, rhamnose and glucuronic acid content. (*: p < 0.05, t-test, n = 4). b Expression analysis of PAGR-YFP in transgenic lines. Protein extracts from 10-day old T3 35S::PAGR-YFP seedlings and from the Wild Type were analyzed by immunoblotting and PAGR-YFP was detected with ‘universal antibody’ against the AttB2 site linker peptide in PAGR-YFP. Coomassie staining of Rubisco large subunit (RBCL) is shown as a loading control

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