Table 5 Sequence analyses of all putative GmGLYII proteins for the presence of conserved motifs and enzyme activity
Sl. no | Proteins | Conserved metal binding motif (THHHXDH) | Active site motif (C/GHT) | Expected GLYII enzyme activity |
|---|---|---|---|---|
1 | GmGLYII-1.1 | Absent | Present | No |
2 | GmGLYII-2.1 | Present | Present | Yes |
3 | GmGLYII-2.2 | Present | Present | Yes |
4 | GmGLYII-2.3 | Present | Present | Yes |
5 | GmGLYII-2.4 | Absent | Present | No |
6 | GmGLYII-3.1 | Present | Present | Yes |
7 | GmGLYII-4.1 | Present | Present | Yes |
8 | GmGLYII-4.2 | Present | Present | Yes |
9 | GmGLYII-5.1 | Present | Present | Yes |
10 | GmGLYII-5.2 | Present | Present | Yes |
11 | GmGLYII-5.3 | Present | Present | Yes |
12 | GmGLYII-6.1 | Present | Present | Yes |
13 | GmGLYII-7.1 | Present | Present | Yes |
14 | GmGLYII-7.2 | Present | Present | Yes |
15 | GmGLYII-8.1 | Present | Present | Yes |
16 | GmGLYII-8.2 | Present | Present | Yes |
17 | GmGLYII-9.1 | Present | Present | Yes |
18 | GmGLYII-9.2 | Present | Present | Yes |
19 | GmGLYII-10.1 | Present | Present | Yes |
20 | GmGLYII-11.1 | Absent | Present | No |
21 | GmGLYII-12.1 | Present | Present | Yes |
22 | GmGLYII-12.2 | Present | Present | Yes |
23 | GmGLYII-12.3 | Present | Present | Yes |