Fig. 9
Effect of exogenous GA on the OsRH2 and OsRH34 double-knockdown T1 transgenic rice. a Three-day-old rice seedlings were incubated in water containing 0, 0.1, and 1 μM GA3 for 7 days. Bars = 1 cm. b A starch plate assay of α-amylase activity. Embryoless half seeds were incubated on starch plates with 10−6 M GA3 for 2 days. c Quantification of clear zone diameter on starch plates with 10−5 and 10−6 M GA3. Error bars show ± SD (n = 40). d Expression of OsGA20ox2 and OsGAMYB in the OsRH2 and OsRH34 double-knockdown seedlings. Total RNAs were isolated from three-leaf-stage seedling and subjected to qRT-PCR. Rice Act1 as an internal control. Error bars indicate the SD of four replicate experiments with two biological replicates. Gene expression was related to wild-type plants, as 1. * is significantly different from the wild-type plants (Student’s t test: p <0.05)