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Fig. 8 | BMC Plant Biology

Fig. 8

From: PtrA/NINV, an alkaline/neutral invertase gene of Poncirus trifoliata, confers enhanced tolerance to multiple abiotic stresses by modulating ROS levels and maintaining photosynthetic efficiency

Fig. 8

Determination of ROS accumulation and antioxidant enzymes activities. a-d In situ accumulation of H2O2 and O2 •- in the transgenic lines and wild type (WT) under normal conditions (a), cold (b), salt (c), and dehydration (d), as revealed by staining with DAB and NBT, respectively. Bars = 1 cm. e-f Quantitative measurement of H2O2 contents before and under cold (e) and salt (f) stress. g-i Enzyme activities of SOD (g), POD (h), and CAT (i) in the transgenic lines and WT before and after cold treatment (n = 3). Statistically significant differences between the transgenic lines and WT under the same conditions are shown (*P < 0.05, **P < 0.01, ***P < 0.001)

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