Fig. 6
Salt stress tolerance assay of potted plants. a Growth phenotype of two-week-old transgenic lines and wild type (WT) before and after spraying with 300 mM NaCl and after a two-week recovery period. Survival rates of each line are shown below the bottom panel (n = 12). b Treatment of potted plants with 300 mM NaCl for 45 days (n = 4), and observation of chlorophyll fluorescence imaging. False colour scale between 0 and 1 is shown below the imaging. c-g Analysis of photosynthesis-related parameters, including Fv/Fm (c), the operating quantum efficiency of the photochemistry (ΦPSII, d), photochemical quenching (qP, e), non-photochemical quenching (NPQ, g), and electron transfer rate (ETR, f), which were recorded using the dark–light induction curve, and displayed using the Imaging WinGegE software (Walz, Effeltrich, Germany). h-i Evans blue staining (h) and MDA level (i) before and after salt stress (n = 3). Bar = 5 cm (a, b) or 1 cm (h). Each experiment was repeated at least twice. Statistically significant differences between the transgenic lines and WT under the same conditions were shown (*P < 0.05, **P < 0.01, ***P < 0.001)