Fig. 3From: PtrA/NINV, an alkaline/neutral invertase gene of Poncirus trifoliata, confers enhanced tolerance to multiple abiotic stresses by modulating ROS levels and maintaining photosynthetic efficiencySubcellular localization of PtrA/NINV in tobacco epidermal cells. GFP (a) or PtrA/NINV::GFP (b-c) was transiently expressed in tobacco epidermal cells and observed under different fields. d is a zoom-up of the stomata in the images of c. GFP fluorescence was observed using the green channel, and mitochondria were visualized by MitoTracker staining using the red channel. Chlorophyll autofluorescence was visualized using UV channel. The right image of panel b is overlaid using the three images on the left. Arrows point to the mitochondria. The right images of (c and d) were obtained by merging the images of GFP and autofluorescence. Bar =10 μmBack to article page