TY - JOUR AU - Jones, Kiersun AU - Kim, Dong Won AU - Park, Jean S. AU - Khang, Chang Hyun PY - 2016 DA - 2016/03/22 TI - Live-cell fluorescence imaging to investigate the dynamics of plant cell death during infection by the rice blast fungus Magnaporthe oryzae JO - BMC Plant Biology SP - 69 VL - 16 IS - 1 AB - Plant cell death plays important roles during plant-pathogen interactions. To study pathogen-induced cell death, there is a need for cytological tools that allow determining not only host cell viability, but also cellular events leading to cell death with visualization of pathogen development. Here we describe a live cell imaging method to provide insights into the dynamics of cell death in rice (Oryza sativa). This method uses live-cell confocal microscopy of rice sheath cells mechanically damaged or invaded by fluorescently-tagged Magnaporthe oryzae together with fluorescent dyes fluorescein diacetate (FDA) and propidium iodide (PI). FDA stains the cytoplasm of live cells exclusively, thus also visualizing the vacuole, whereas PI stains nuclei of dead cells. SN - 1471-2229 UR - https://doi.org/10.1186/s12870-016-0756-x DO - 10.1186/s12870-016-0756-x ID - Jones2016 ER -