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Fig. 4 | BMC Plant Biology

Fig. 4

From: Effect of atmospheric carbon dioxide levels and nitrate fertilization on glucosinolate biosynthesis in mechanically damaged Arabidopsis plants

Fig. 4

Foliar glucosinolate levels in Arabidopsis grown at different levels of CO2, nitrate fertilization and wounding stress. Plants were grown under ambient (440 ppm) or elevated (880 ppm) carbon dioxide (CO2) levels and fertilized with either 1 mM or 10 mM nitrate (1 mM N or 10 mM N). At 6 weeks, rosette leaves were mechanically damaged (Mech damaged) or untouched (control, C). a Total glucosinolates, white bars represent aliphatic GSLs and hatched bars represent indole GSLs. Significant differences (p ≤ 0.05) in response to wounding of aliphatic GSLs are denoted inside the white bar, of indole GSLs are denoted inside the hatched bar and total GSLs are denoted on top of the bar by alphabetical letters. b Aliphatic glucosinolates Bi) Glucoiberin (IBE; 3-methylsulfinylpropyl GSL), Bii) Glucoerucin (ERU; 4-methylthiobutyl GSL), Biii) Glucoraphanin (RAPH; 4-methylsulfinylbutyl GSL) and Biv) d) Glucoalyssin (ALY; 5-methylsulfinylpentyl GSL). c Indole glucosinolates Ci) Glucobrassicin (GBC), Cii) Neo-glucobrassicin (NeoGB) and Ciii) Methoxyglucobrassicin (MeOGB). Statistical differences were determined by 3-factor analysis of variance (Additional file 4: Table S3). When interactions were significant, data were separated to show treatment effects. Significant differences in response to wounding are denoted by alphabetical letters (p ≤ 0.05). An asterisk indicates significant differences between grouped variables

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