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Table 4 Proteins that varied in abundance in response to cold acclimation in a single genetic background

From: A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

Cultivar

Cold regulation

Spot no.

Homology

Function

Average CA/NA protein ratio

TF0

TF3

TF4

Average

Christie

Increased

1560

Eukaryotic translation initiation factor 5A-like

Transcription and translation

4.8

8.12

10.01

7.64

Decreased

1683

Peroxiredoxin

Response to stress (ROS scavenging)

−3.17

−3.79

−2.34

−3.1

Endure

Increased

74

Aconitate hydratase

Carbohydrate and energy metabolism (TCA cycle)

2.25

2.23

3.39

2.62

Increased

18

Nuclease domain-containing protein

Transcription and translation (RNA stability)

2.21

2.44

2.76

2.47

Increased

595

Elongation factor 1-gamma

Transcription and translation

2.52

2.14

2.52

2.39

Increased

92

Elongation factor 2-like

Transcription and translation

2.32

2.06

2.19

2.19

Increased

64

Puromycin-sensitive aminopeptidase-like

Protein metabolism

2.14

1.88

1.7

1.91

Increased

586

Elongation factor 1-gamma

Transcription and translation

2.06

1.82

1.59

1.82

Increased

594

Elongation factor 1-gamma-like

Transcription and translation

1.77

1.81

1.6

1.73

Increased

68

Peptidase M1 family aminopeptidase N

Protein metabolism

1.85

1.57

1.52

1.65

Decreased

1732

Disease resistance response protein 1

Response to stress (defense, PR protein)

−3.31

−1.81

−2.99

−2.70

  1. Cold-acclimated (CA) to non-acclimated (NA) protein abundance that statistically differed (Student's t-test, P < 0.01) and with a CA/NA ratio ≥ |1.5| in all populations of the genetic background are presented. TF0 = original genetic background; TF3 and TF4 = populations produced after three and four cycles of selection for freezing tolerance, respectively