A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

BMC Plant Biology

Table 3 DIGE-spots with homology with sequences in databases that were down-regulated in response to cold acclimation

Spot no.	Homology	Functional category	CA/NA ratio
130	Sucrose synthase	Carbohydrate and energy metabolism	−3.51
545	Tubulin alpha	Signal transduction	−3.40
1660	Glycine-rich RNA-binding protein	Response to stress	−3.14
1325	6-phosphogluconolactonase	Carbohydrate and energy metabolism	−2.65
169	Methionine synthase	Amino acid metabolism	−2.57
1182	14-3-3-like protein	Signal transduction	−2.48
471	ATP synthase subunit beta	Carbohydrate and energy metabolism	−2.46
1694	Pathogenesis-related protein 10a	Response to stress (Defense, PR protein)	−2.39
605	Elongation factor 1-alpha-like	Transcription and translation	−2.39
700	Actin-101-like	Signal transduction	−2.35
1310	Thaumatin-like protein	Response to stress (Defense, PR protein)	−2.23
2037	Isoflavone reductase family protein	Response to stress	−1.84

For each spot, the name of the homologous sequence, the functional category and the average CA/NA abundance ratio across the six populations are listed. Spots with cold acclimated (CA) to non-acclimated (NA) abundance ratio ≤− 1.5 and with t-test, P < 0.05 in at least five populations are presented. In cases when homologous sequences were detected at different positions on the gels, the spot with the lowest CA/NA ratio is presented. The exhaustive list of polypeptides up-regulated in response to cold acclimation is provided in Additional file 4: Table S4b

ISSN: 1471-2229