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Table 3 DIGE-spots with homology with sequences in databases that were down-regulated in response to cold acclimation

From: A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

Spot no. Homology Functional category CA/NA ratio
130 Sucrose synthase Carbohydrate and energy metabolism −3.51
545 Tubulin alpha Signal transduction −3.40
1660 Glycine-rich RNA-binding protein Response to stress −3.14
1325 6-phosphogluconolactonase Carbohydrate and energy metabolism −2.65
169 Methionine synthase Amino acid metabolism −2.57
1182 14-3-3-like protein Signal transduction −2.48
471 ATP synthase subunit beta Carbohydrate and energy metabolism −2.46
1694 Pathogenesis-related protein 10a Response to stress (Defense, PR protein) −2.39
605 Elongation factor 1-alpha-like Transcription and translation −2.39
700 Actin-101-like Signal transduction −2.35
1310 Thaumatin-like protein Response to stress (Defense, PR protein) −2.23
2037 Isoflavone reductase family protein Response to stress −1.84
  1. For each spot, the name of the homologous sequence, the functional category and the average CA/NA abundance ratio across the six populations are listed. Spots with cold acclimated (CA) to non-acclimated (NA) abundance ratio ≤− 1.5 and with t-test, P < 0.05 in at least five populations are presented. In cases when homologous sequences were detected at different positions on the gels, the spot with the lowest CA/NA ratio is presented. The exhaustive list of polypeptides up-regulated in response to cold acclimation is provided in Additional file 4: Table S4b