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Table 2 DIGE-spots with homology with sequences in databases that were up-regulated in response to cold acclimation

From: A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

Spot no. Homology Functional category CA/NA ratio
2039 Cold responsive protein TRVSP Response to stress 29.54
1648 KS-dehydrin Response to stress 20.81
2051 Cold acclimation-specific protein Response to stress 11.03
1776 Actin-depolymerizing factor 2-like Signal transduction 9.43
1601 Peptidyl-prolyl cis-trans isomerase 1-like Molecular chaperones and protein folding 5.92
1587 Phospholipid hydroperoxide glutathione peroxidase Response to stress (ROS scavenging) 5.14
547 Enolase-like Carbohydrate and energy metabolism 5.02
93 Elongation factor 2-like Transcription and translation 4.37
353 2,3-bisphosphoglycerate-independent phosphoglycerate mutase Carbohydrate and energy metabolism 4.26
2046 Tyrosine phosphatase Signal transduction 4.10
802 Monodehydroascorbate reductase Response to stress (ROS scavenging) 4.07
142 Methionine synthase Amino acid metabolism 4.05
1711 Ubiquitin-conjugating enzyme Molecular chaperones and protein folding 3.92
318 NADP-dependent malic enzyme Carbohydrate and energy metabolism 3.57
810 3-hydroxyisobutyryl-CoA hydrolase-like Signal transduction (Cold stress signalling) 3.45
1365 Glutathione S-transferase Response to stress (ROS scavenging) 3.38
1286 Mitochondrial outer membrane protein porin Metabolite transport 3.32
887 Glyceraldehyde-3-phosphate dehydrogenase Carbohydrate and energy metabolism 3.32
1280 Outer plastidial membrane protein porin-like Metabolite transport 3.29
278 Stress-induced-phosphoprotein Signal transduction 3.17
760 Transaldolase-like Carbohydrate and energy metabolism 3.12
598 Elongation factor 1-gamma Transcription and translation 3.08
378 Protein disulfide isomerase-like protein precursor Molecular chaperones and protein folding 2.98
1510 Universal stress protein A-like protein Response to stress 2.95
664 Abscisic acid stress ripening protein Signal transduction 2.89
1369 Ferritin-2 Response to stress (ROS scavenging) 2.88
458 Adenosylhomocysteinase-like Amino acid metabolism 2.87
1095 Isoflavone reductase related protein Response to stress (ROS scavenging) 2.73
619 Elongation factor 1-alpha-like Transcription and translation 2.65
66 Aconitate hydratase Carbohydrate and energy metabolism 2.55
814 Fructose-bisphosphate aldolase Carbohydrate and energy metabolism 2.40
2048 Malate dehydrogenase Carbohydrate and energy metabolism 2.35
694 Isocitrate dehydrogenase [NADP] Carbohydrate and energy metabolism 2.30
283 Heat shock protein Molecular chaperones and proteins folding 2.20
842 Adenosine kinase Carbohydrate and energy metabolism 2.18
1267 Proteasome subunit alpha type Molecular chaperones and protein folding 2.00
527 Phosphopyruvate hydratase Carbohydrate and energy metabolism 1.89
41 2-oxoglutarate dehydrogenase E1 subunit-like Carbohydrate and energy metabolism 1.89
792 Glutamine synthetase Amino acid metabolism 1.86
939 Quinone oxidoreductase-like protein Response to stress (ROS scavenging) 1.81
772 Aspartate aminotransferase Amino acid metabolism 1.81
1430 Chalcone isomerase 2 Response to stress (ROS scavenging) 1.80
769 Formate dehydrogenase 1 Carbohydrate and energy metabolism 1.78
667 Citrate synthase-like protein Carbohydrate and energy metabolism 1.74
971 Fructokinase-2-like Carbohydrate and energy metabolism 1.73
580 6-phosphogluconate dehydrogenase, decarboxylating-like Carbohydrate and energy metabolism 1.70
  1. For each spot, the name of the homologous sequence, the functional category and the average CA/NA abundance ratio across the six populations are listed. Spots with cold acclimated (CA) to non-acclimated (NA) abundance ratio ≥ 1.5 and with t-test, P < 0.05 in at least five populations are presented. In cases when homologous sequences were detected at different positions on the gels, the spot with the highest CA/NA ratio is presented. The exhaustive list of polypeptides up-regulated in response to cold acclimation is provided in Additional file 3: Table S4a