Fig. 3

Phylogeny, CXC pattern and tissue EST expression of the Medicago truncatula PA1 paralogues. Unrooted Bayesian tree of Medicago truncatula PA1 family (53 sequences, 366 nucleic acid positions). The tree is presented according to rooted phylogeny shown on Fig. 4. Numbers at branch correspond respectively to posterior probabilities calculated with MrBayes, and to bootstrap values estimated by PhyML. The scale bar represents the average number of substitutions per site. Six strongly supported clusters were boxed and highlighted with colored backgrounds. The seven chemically and functionally synthetized sequences (AG41, EG41, GL44, AS40, AS37, DS37 and QT41) are indicated on the tree. Among them, AG41, EG41, AS40, and AS37 (boxed in purple), and showed toxicity against insect cells, whereas GL44, DS37 and QT41 (boxed in light green) did not show toxicity to insect cells. In the tissue expression part, the color-scale represent the expression value scales between 0 and >75 EST per cluster. Two internal sub-clusters were defined for running site model in cluster 3 and 6, and are named respectively 3a, 3b, and 6a, 6b (Table 2). Red branches are those that were tested for positive selection (Table 2)