Fig. 2

RT-qPCR validation of differentially expressed genes (DEGs). RT-qPCR results of the pattern of gene expression between bruchid-resistant and -susceptible mungbean. The Y axis indicates the relative quantity (RQ) of gene expression with mungbean VrActin (g12676) used as a control. Data are RQ ± SE of ΔΔCT from three experimental repeats. The X axis indicates different bruchid-resistant (R) and -susceptible (S) mungbean lines. Asterisk indicates that the expression of the gene was not detected in the parental line NM92 with CT value set to 40 cycles for calculating the RQ of gene expression