Fig. 5From: Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinasesSubstrate Preference of MPK3/4/6. a Thiophosphorylation assays of native, FLAG-tagged AT1G78150, AT2G26530, AT3G11330 and AT4G38710 in the presence of Bn-ATPγS as cofactor and using either AS-MPK3/4/6 or Wt-MPK3/4/6 as a negative control. b Same as in (a) but using E. coli expressed T7-tagged MKS1 as a substrate. Sample wells that were left empty are indicated by an asterisk (*)Back to article page