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Table 2 Representative significant enriched GO terms of the differential expressed unigenes

From: Exogenous auxin regulates multi-metabolic network and embryo development, controlling seed secondary dormancy and germination in Nicotiana tabacum L.

Seed status

Category

GO term

Refence set

Query set

P-value (FDR)

Representative gene ID

Encoded protein

Dormancy

BP

Para-aminobenzoic acid metabolic process

130

19

2.20E-04

TCONS_00105446

glucosyltransferase

Oxalate catabolic process

6

4

2.99E-03

TCONS_00017169

4-coumarate--CoA ligase-like 10-like

N-acylethanolamine metabolic process

34

9

6.41E-04

TCONS_00047595

fatty acid amide hydrolase-like

Glutamate biosynthetic process

25

7

2.99E-03

TCONS_00068711

glutamate synthase 1

Gibberellin metabolic process

127

13

3.21E-02

TCONS_00065700

gibberellin 2-oxidase 2,3,5

Flavonol biosynthetic process

109

13

1.15E-02

TCONS_00051510

immediate-early salicylate-induced glucosyltransferase

Cutin biosynthetic process

47

9

3.95E-03

TCONS_00032450

CYP77A19

Auxin mediated signaling pathway

548

43

8.41E-04

TCONS_00056392,TCONS_00049526,TCONS_00102987,TCONS_00043746,TCONS_00029963,TCONS_00082867

NTGP3, Nt-iaa4.5 deduced protein, IAA9, ARF1, IAA13, LAX2

MF

Adenylyl-sulfate reductase activity

5

4

1.43E-03

TCONS_00030231

APS reductase

Carbon-nitrogen ligase activity, with glutamine as amido-N-donor

200

19

1.85E-02

TCONS_00077280

amidase-like protein

Glutamate synthase (NADH) activity

22

8

3.01E-04

TCONS_00068708

glutamate synthase 1

Indole-3-acetic acid amido synthetase activity

42

8

1.43E-02

TCONS_00011340

Nt-gh3 deduced protein

Isoflavone 2′-hydroxylase activity

21

5

4.94E-02

TCONS_00066756

cytochrome P450

Oxalate-CoA ligase activity

6

4

3.69E-03

TCONS_00017169

4-coumarate--CoA ligase-like 10-like

Phosphoadenylyl-sulfate reductase (thioredoxin) activity

5

4

1.43E-03

TCONS_00030231

APS reductase

Germiantion

BP

ABA mediated signaling pathway

547

35

3.19E-03

TCONS_00022716, TCONS_00055623, TCONS_00066091

bZIP, LEB5, calcium-dependent protein kinase 8

Auxin mediated signaling pathway

548

34

5.43E-03

TCONS_00033126, TCONS_00075562, TCONS_00084884

auxin efflux facilitator PIN3b, Nt-iaa28 deduced protein, germin like protein

Cinnamic acid biosynthetic process

16

7

2.95E-05

TCONS_00069919

phenylalanine ammonia-lyase 4

Cysteine biosynthetic process

547

34

5.31E-03

TCONS_00016976, TCONS_00018745, TCONS_00017791

serine acetyltransferase 7, chloroplast pigment-binding protein CP24, ZIP

Ethylene metabolic process

247

20

4.37E-03

TCONS_00086037, TCONS_00008535, TCONS_00106286

ethylene forming enzyme,WRKY transcription factor NtEIG-D48, Avr9/Cf-9 rapidly elicited protein 74

JA mediated signaling pathway

592

38

1.90E-03

TCONS_00016976, TCONS_00057641,TCONS_00052052, TCONS_00055412, TCONS_00081296

serine acetyltransferase 7, BOP3, WRKY DNA-binding protein, jasmonate ZIM-domain protein10, MAP kinase kinase

L-phenylalanine catabolic process

50

7

1.79E-02

TCONS_00069919

phenylalanine ammonia-lyase 4

Response to blue light

372

25

9.11E-03

TCONS_00051968

chloroplast FtsZ-like protein

Response to far red light

324

24

4.19E-03

TCONS_00033254

chlorophyll a/b-binding protein

Response to red light

285

26

1.38E-04

TCONS_00049275

alpha-expansin precursor

SA mediated signaling pathway

732

42

5.65E-03

TCONS_00081296, TCONS_00055623, TCONS_00016976

MAP kinase kinase, LEB5, serine acetyltransferase 7

MF

Phenylalanine ammonia-lyase activity

15

7

1.13E-05

TCONS_00069919

phenylalanine ammonia-lyase 4

Serine-type endopeptidase inhibitor activity

34

10

4.64E-06

TCONS_00053724, TCONS_00009793

trypsin proteinase inhibitor precursor, cyclin-T1-3-like

UDP-glucosyltransferase activity

581

39

4.20E-04

TCONS_00002352, TCONS_00039739, TCONS_00066391

glucosyltransferase, UDP-glucose, SA glucosyltransferase, flavonoid 3-O-glucosyltransferase

CC

Chloroplast thylakoid membrane

1211

91

5.07E-14

TCONS_00032434, TCONS_00018745, TCONS_00051968

plastid transketolase, chloroplast pigment-binding protein CP24, chloroplast FtsZ-like protein

Photosystem I reaction center

28

9

1.81E-06

TCONS_00002104, TCONS_00102030, TCONS_00067457

PSI-H precursor, photosystem I subunit XI, PSI-H precursor

Photosystem II reaction center

6

4

1.91E-04

TCONS_00065835, TCONS_00027279

photosystem II protein T, photosystem II reaction center PSB28 protein