Skip to main content
Fig. 7 | BMC Plant Biology

Fig. 7

From: Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana

Fig. 7

Analysis of dolichol-linked oligosaccharides. a HPLC-based size fractionation of 2-pyridylamine (PA)-labelled oligosaccharides after acid hydrolysis and purification of the lipid-linked oligosaccharides from the indicated yeast strains. Inserts depict the deduced structures of peaks 1/4 and peaks 2/3 (as described below in b and c). b Structure determination by LC-MS/MS of peaks 1–4. c RP-HPLC comparison of peaks 1–4 to known oligosaccharide standards. Symbols depicting oligosaccharide structures are as defined in A. *For A and C, low levels of contaminants in the HPLC traces are marked with asterisks. (Abbreviations: G.U. = Glucose oligomer unit, Glc = Glucose, GlcNAc = N-Acetylglucosamine)

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us