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Fig. 5 | BMC Plant Biology

Fig. 5

From: Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana

Fig. 5

The cce2/cce3 mutants are allelic to ALG3. a A T-DNA insertion alg3 mutant (alg3-T, SALK_064006) was crossed to cce2 and cce3, successful crosses were validated by PCR in the F1, and the flg22-induced calcium response was monitored in the segregating F2 seedlings (marked with red broken lines). Error bars denote standard deviations (n =96 for pMAQ2, cce2 or cce3; n = 66 for cce2xalg3-T, n = 49 for cce3xalg3-T). The same pMAQ2 (“wild type”) reference calcium curve was used for both graphs. Calcium signatures were tested for statistical significance between genotypes by one-way ANOVA (with Tukey’s multiple comparison test, p < 0.05). b After identifying homozygous alg3-T individuals (from the cce2xalg3-T cross with the indicated primer pairs by PCR of genomic DNA, right panel), flg22-induced calcium response was monitored as above except leaf disks (Ø 4 mm) of 4-week-old plants were used (n = 96 for alg3-T; n =32 for pMAQ2 or cce2). Calcium elevations in cce2 and alg3-T are not different but are both statistically distinct from the pMAQ2 control (one-way ANOVA with Tukey’s multiple comparison test, p < 0.01)

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BMC Plant Biology

ISSN: 1471-2229

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