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Fig. 3 | BMC Plant Biology

Fig. 3

From: Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus

Fig. 3

OsWAK91 and OsWAK112d over-expresser lines are affected for M. oryzae resistance. Expression of OsWAK91 and OsWAK112d was measured by quantitative RT-PCR in non-infected lines over-expressing (OE) OsWAK91 (a) or OsWAK112d (b) genes and the corresponding null-segregant lines (NS). Gene expression, calculated from three biological replicates, was normalized using Actin. The fold change between NS and OE is also indicated (a and b). Plants were inoculated with the virulent isolate GY11 of M. oryzae. The total number of lesions was counted as in Fig. 2 in the lines OE-OsWAK91 (c) or OE-OsWAK112d (d). For each mutant line, the average number of lesions over more than 7 plants was calculated for the corresponding over-expresser and null-segregant line. This value was used to calculate the percentage of lesions per individual mutant plant as compared to the mean of the null-segregant plants. The mean and standard deviation was then calculated. This experiment was repeated three times and one representative experiment is shown. A t-test was done to evaluate the significance of the observed differences (*: p <0.05; **: p <0.001). Pictures of typical symptoms on over-expresser and null-segregant lines were taken 5 days post inoculation (e and f). Several other over-expresser lines were also produced and analyzed (Additional file 4)

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