Fig. 1

OsWAK gene expression during infection and upon chitin treatment. WAK gene expression was measured by quantitative RT-PCR in leaf tissues under inoculation by M. oryzae (a), after chitin treatment (b) and in the cebip mutant (c). The data were normalized using Actin and all values shown are expressed as Arbitrary Units. For OsWAK112d, the two alternative transcripts described (Additional file 3A) gave the same expression pattern and the longest one is shown. Mean values are provided with the standard error (n = 4). Statistical differences were evaluated according to one-way ANOVA followed by Dunnett’s test relative to Mock condition for each data point P <0.05 (*), P <0.01 (**) and P <0.001 (***). For panel (c), only significant tests between wild-type and cebip mutants treated with chitin are shown. a Plants inoculated with gelatin only (Mock treatment: white bars) or with Magnaporthe oryzae (virulent isolate FR13: dark bars; avirulent isolate CL367: grey bars) at different hours after treatment. b Chitin and water were sprayed on rice plants. The values are the mean calculated from four independent biological replicates (white bars: mock; light grey bars: 100 μg/mL chitin; dark grey bars: 1000 μg/mL chitin). c Regulation of OsWAK genes after chitin treatment (continous lines, 1000 μg/mL chitin) or mock-treated (dashed lines) in the cebip mutant (grey lines) and the corresponding null-segregant (wild-type) plants (black lines)