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Fig. 6 | BMC Plant Biology

Fig. 6

From: OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress

Fig. 6

Expression profile of OsHKT1;4 in various tissues of Nipponbare rice plants. Quantitative real-time PCR analyses were performed using RNA samples derived from various tissues. The expression of OsHKT1;4 and an internal control OsSMT3 was determined. The level of OsHKT1;4 expression was normalized using OsSMT3 expression. a, Relative expression of OsHKT1;4 in tissues of hydroponically grown Nipponbare plants in the vegetative growth phase (3-weeks-old) with or without 50 mM NaCl treatments for 3 days is shown setting its expression in the basal node without stress to 1 (n = 6, ±SD). LB6: 6th leaf blade; LB5: 5th leaf blade; LS6: 6th leaf sheath; LS5: 5th leaf sheath; BN: basal node; R: root. b, Relative expression of OsHKT1;4 in tissues of soil-grown Nipponbare plants in the reproductive growth phase with or without NaCl treatments (25–100 mM) for more than 1 month is shown setting its expression in the flag leaf sheath to 1 (n = 5-6, ±SD). FLB: flag leaf blade; FLC: flag leaf sheath; P: peduncle; N I: node I; IN II: internode II; N II: node II. Note that insets in a and b show the data sets from some tissues in a smaller scale than that in the main graphs. c, Relative OsHKT1;4 expression in enlarged vascular bundles (EBVs) of node I, diffuse vascular bundles (DVBs) of node I, and the basal stem (BS) is shown setting its expression in the basal stem to 1 (n = 6, ±SD). EVBs and DVBs were excised from node I by laser microdissection (LMD). N.D. indicates “not detected”. The Welch’s-t test was used for the statistical analysis: * P < 0.05, ** P < 0.01, *** P < 0.001 vs. no stress condition (a, b) or basal stem (c)

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