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Fig. 5 | BMC Plant Biology

Fig. 5

From: Salicylic acid treatment and expression of an RNA-dependent RNA polymerase 1 transgene inhibit lethal symptoms and meristem invasion during tobacco mosaic virus infection in Nicotiana benthamiana

Fig. 5

Western blot analysis for MtRDR1 in RDR1-enriched protein extracts. Leaves of empty vector control transgenic or MtRDR1-transgenic N. benthamiana plants were infiltrated with a control solution of water containing 0.05 % (v/v) ethanol (-) or 2.5 mM SA (+). RDR1-enriched protein extracts were prepared 48 h later and subjected to western blot analysis with either preimmune rabbit serum (left panel) or polyclonal rabbit anti-MtRDR1 serum (right panel). The positions of protein molecular mass markers are indicated (kDa). A protein (indicated with a star) corresponding to the predicted size of 131 kDa for MtRDR1 was specifically detected in SA-treated MtRDR1-transgenic plants with the anti-MtRDR1 serum but not with the preimmune serum. Equal loading of gel lanes with protein, based on the Bradford [59] assay was confirmed by Ponceau S staining of the western blot membrane (lower panels)

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