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Fig. 8 | BMC Plant Biology

Fig. 8

From: A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog

Fig. 8

Transcript abundance of different alleles and DNA methylation in the FT2a genomic region. a Transcript abundance of different FT2a alleles assayed by allele-specific restriction digestion. b Diagram of the FT2a genomic region showing the position of SORE-1 insertion. Amplicons were analyzed by semi-quantitative PCR after McrBC or mock digestion; the amplified regions are designated as a to k and S1 to S4. Exons, white; UTRs, black; LTRs of SORE-1, gray. c Genomic DNA from leaves of 20-day-old plants of NILs for FT2a-TO (TO) and FT2a-HY (HY) grown under SD conditions was digested with McrBC (+) or mock-digested (–) and amplified by PCR. Amplicons were visualized in agarose gels

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