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Table 2 Gametophytic chromosome quantification using meiotic spreads and pWOX2-CENH3-GFP

From: CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Ploidy Method n   Microspore frequency (%)      
     # Chromosomes
     1 2 3 4 5 6 7 8 9
Diploid MII 72 Mean 0.0 0.0 0.0 0.0 100.0 0.0 0.0 0.0 0.0
    range -- -- -- -- 0.0 -- -- -- --
  CENH3 531 Mean 0.0 0.0 0.9 12.7 86.3 0.0 0.0 0.0 0.0
    SD -- -- 1.3 6.5 7.6 -- -- -- --
     # Chromosomes
     6 7 8 9 10 11 12 13 14
Tetraploid MII 66 Mean 0.0 0.4 2.7 9.9 78.8 8.0 0.4 0.0 0.0
    range -- 0.6 3.7 6.4 6.4 3.7 0.6 -- --
  CENH3 238 Mean 0.0 1.7 7.8 15.6 64.4 8.4 1.7 0.3 0.0
    SD -- 1.4 2.6 1.8 2.7 2.9 1.4 0.6 --
     # Chromosomes
     3 4 5 6 7 8 9 10 11
Triploid MII 52 Mean 0.0 1.1 5.4 13.1 30.5 30.5 13.1 5.4 1.1
    range -- 1.6 3.1 3.2 3.2 3.2 3.2 3.1 1.6
  CENH3 732 Mean 0.0 0.5 6.6 15.3 31.3 29.9 11.2 2.4 0.4
    SD -- 0.7 0.6 4.8 3.5 2.5 3.9 2.8 0.9
  1. Distribution of the absolute number of chromosomes in male gametes resulting from MII meiotic chromosome segregation analysis and distribution of centromeric GFP dots in uninuclear microspores of diploid, triploid, and tetraploid Arabidopsis pWOX2-CENH3-GFP lines. For triploid meiosis, data from maternal and paternal excess plants was combined. In triploid and tetraploid meiosis, the occurrence of lagging chromosomes in MI (resulting in polyads; respectively 4.4 and 9 %) was integrated in the predicted ploidy distribution of the resulting gametes. As lagging chromosomes are not always detected in MII meiotic spreads (e.g. due to accidental polar localization by performing the chromosome spread), the frequency of polyad figures, obtained by or orcein-stained tetrad analysis, was used to determine the level of chromosome missegregation and forms the basis for the frequency ranges described
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