Table 2 Gametophytic chromosome quantification using meiotic spreads and pWOX2-CENH3-GFP
From: CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana
| Ploidy | Method | n | Microspore frequency (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| # Chromosomes | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||||
| Diploid | MII | 72 | Mean | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| range | -- | -- | -- | -- | 0.0 | -- | -- | -- | -- | |||
| CENH3 | 531 | Mean | 0.0 | 0.0 | 0.9 | 12.7 | 86.3 | 0.0 | 0.0 | 0.0 | 0.0 | |
| SD | -- | -- | 1.3 | 6.5 | 7.6 | -- | -- | -- | -- | |||
| # Chromosomes | ||||||||||||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||||
| Tetraploid | MII | 66 | Mean | 0.0 | 0.4 | 2.7 | 9.9 | 78.8 | 8.0 | 0.4 | 0.0 | 0.0 |
| range | -- | 0.6 | 3.7 | 6.4 | 6.4 | 3.7 | 0.6 | -- | -- | |||
| CENH3 | 238 | Mean | 0.0 | 1.7 | 7.8 | 15.6 | 64.4 | 8.4 | 1.7 | 0.3 | 0.0 | |
| SD | -- | 1.4 | 2.6 | 1.8 | 2.7 | 2.9 | 1.4 | 0.6 | -- | |||
| # Chromosomes | ||||||||||||
| 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | ||||
| Triploid | MII | 52 | Mean | 0.0 | 1.1 | 5.4 | 13.1 | 30.5 | 30.5 | 13.1 | 5.4 | 1.1 |
| range | -- | 1.6 | 3.1 | 3.2 | 3.2 | 3.2 | 3.2 | 3.1 | 1.6 | |||
| CENH3 | 732 | Mean | 0.0 | 0.5 | 6.6 | 15.3 | 31.3 | 29.9 | 11.2 | 2.4 | 0.4 | |
| SD | -- | 0.7 | 0.6 | 4.8 | 3.5 | 2.5 | 3.9 | 2.8 | 0.9 | |||
