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Fig. 3 | BMC Plant Biology

Fig. 3

From: CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Fig. 3

Gamete-specific centromere labeling in female pWOX2-CENH3-GFP embryo sacs. a-t Bright field imaging and GFP-based assessment of pWOX2-CENH3-GFP expression and localization during female reproduction; pre-meiotic stage a and b, meiosis e and f, uninuclear megaspore i and j, binuclear embryo sac m and n and fully matured seven-celled embryo sac q and r. Arrows indicate gametophytic cells. Scale bar, 20 μm. Corresponding stages in male gametophytic development are depicted next to each ovule figure; uninuclear d and e, binuclear microspore g and h and trinuclear k, l, o, p, s and t pollen stage. Scale bar, 10 μm. Images are single snapshots and hence not all centromeric signals may be displayed

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