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Fig. 2 | BMC Plant Biology

Fig. 2

From: CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Fig. 2

CENH3-GFP driven by pWOX2 labels centromeres in male gametogenesis. a-h Expression and centromere-specific localization of the pWOX2-CENH3-GFP protein during male gametophyte development; meiotic tetrad stage a, early uninuclear microspore b, mid-uninuclear microspore c, late uninuclear microspore d and e, first mitotic division with doublet CENH3-GFP dots f, binuclear microspore stage g and h. Images are processed z-stack files. Bright fluorescent dots in a and b are also observed in wild type. Scale bar, 10 μm. i Frequency distribution of male spores (n = 383) depending on the number of CENH3-GFP fluorescent dots at the late uninuclear microspore stage. Error bars represent standard deviation (of three independent analyses of at least 100 spores)

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