Fig. 1

Modulating CENH3-GFP expression in Arabidopsis using different promoters. a-f Bright field and GFP fluorescent imaging in wild type PMCs showing autofluorescent dots at meiotic initiation a and d, prophase I b and e and tetrad stage c and f. g-r Promoter-dependent expression and localization of CENH3-GFP in somatic and reproductive organs in Arabidopsis. g pASY2-CENH3-GFP fluorescent dots in uninuclear microspores. h-j Expression of pAtPS1- and p35S-CENH3-GFP in respectively roots h and leaves i of ten-day-old seedlings. Scale bars, 10 μm. j pAtPS1-CENH3-GFP expression in mature petals. Scale bar, 50 μm. k-l GFP fluorescent imaging of pAtPS1-CENH3-GFP premeiotic cells in male sporogenesis. m-r GFP fluorescence in p35S-CENH3-GFP hypocotyls m-n, flower meristems o-p and young petals q-r. Scale bars, 10 μm, except for hypocotyls; 25 μm